鸭LXRα基因过表达对原代肝细胞及PC3细胞脂质代谢的效应研究

doi:10.11843/j.issn.0366-6964.2019.01.007

Abstract

Abstract:

To explore the regulation mechanism of duck LXRα gene on fat deposition,in this experiment,the pEGFP-N3 was used as the vector to construct the LXRα gene eukaryotic over-expression vector carried HIS-tag that was transfected into the duck primary hepatocytes and PC3 cells, respectively. The over expression level of LXRα gene in duck hepatocytes and PC3 cells were detected by the duck HIS-tag kit,and the correlation between the over expression of LXRα gene in the two kinds of cells and the lipid index contents was analyzed,and the regulation mechanism of LXRα gene on lipid metabolism in duck hepatocytes and PC3 cells was explored. The results showed that the over expression of LXRα gene in both cells were significantly positively correlated with the contents of triglyceride (TG) and high-density lipoprotein (HDL) (P<0.01),and significantly negatively correlated with the contents of total cholesterol (TC) and low density lipoprotein (LDL) (P<0.01). The results reveal that over expression of LXRα gene has a positive regulatory effect on the anabolism of TG and HDL,and has a negative regulatory effect on TC and LDL,and the regulatory mechanisms in the two types of cells are similar. The results of this study will lay a theoretical foundation for the further study of the role of LXRα gene in the lipid metabolism network pathway and the solve of excessive fat deposition in duck body.

Key words: duck, LXRα gene, primary hepatocytes, PC3 cells, lipid metabolism

CLC Number:

S834.2

LUO Hualun, LI Wangui, ZHANG Yiyu, WU Lei, QIN Yuanyu. Effect of Duck LXRα Gene Overexpression on Lipid Metabolism in Primary Hepatocytes and PC3 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 52-60.

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Effect of Duck LXRα Gene Overexpression on Lipid Metabolism in Primary Hepatocytes and PC3 Cells

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