ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2019, Vol. 50 ›› Issue (1): 44-51.doi: 10.11843/j.issn.0366-6964.2019.01.006

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Abnormal Expression of LINC24065 in Somatic Cell Nuclear Transfer Cattle

ZHANG Cui1, CHEN Weina1,2, LI Junliang1, GU Shukai1, XU Da1, LI Dongjie3, LI Shijie1*   

  1. 1. College of Life Sciences, Hebei Agricultural University, Baoding 071001, China;
    2. College of Traditional Chinese Medicine, Hebei University, Baoding 071002, China;
    3. College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
  • Received:2018-05-14 Online:2019-01-23 Published:2019-01-24

Abstract:

The objective of this study was to analyze the expression profile and imprinting status of a novel long noncoding RNA (LINC24065) located in the DLK1-DIO3 imprinted domain in natural reproduction(NR) and somatic cell nuclear transfer (SCNT) calves, which would provide a theoretical basis for further understanding the role of DLK1-DIO3 imprinted domain in the reprogramming of SCNT. The brain of NR calves was used as experimental material, a novel long intergenic non-coding RNA was identified by RACE and RT-PCR methods and named LINC24065. The expression and imprinting status of LINC24065 in NR and SCNT calves were analyzed by SNP-based direct sequencing of RT-PCR product. Sequence analysis revealed that LINC24065 contained 6 splicing variants which were expressed in 7 detected tissues of NR calves. But in SCNT calves, LINC24065-V3 was not detected and the other 5 isoforms showed tissue-specific expression. Imprinting status analysis indicated that LINC24065 exhibited monoallelic expression in 7 tissues of NR and SCNT calves; but in brain of SCNT calves, the monoallelic expression of LINC24065 had different parent origin from that in other tissues. The aberrant expression patterns and imprinting status of LINC24065 occurred in SCNT calves. The expression of splicing variants also changed in tissues. These results suggest that LINC24065 gene is associated with abnormal development of SCNT cattle.

Key words: DLK1-DIO3 imprinted domain, epigenetic reprogramming, SCNT, lncRNA

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