毛序棘豆内生真菌分离鉴定及系统进化分析

doi:10.11843/j.issn.0366-6964.2018.09.024

Abstract

Abstract:

To explore the species and population distribution of endophytic fungi from Oxytropis trichophora, Oxytropis trichophora samples in Tongwei county of Gansu province were collected, then were sterilized by using the method of surface disinfection. After that, endophytic fungi were insolated, and then the morphology techniques and ITS sequence analysis were used to identify their species, The phylogenetic relationship of endophytic fungi was analyzed by MEGA 5.05 software. The results showed that 29 species of endophytic fungi were isolated from Oxytropis trichophora belonging to 4 classes, 5 orders, 6 families and 10 genera, the isolation rate of stem was the highest (39.13%), followed with root (23.91%) and leaf was the least (11.11%). Alternaria sp. and Fusarium sp. were the dominant species of endophytic fungi, relative frequency (RF) were 27.59% and 17.24%, respectively. Trichoderma sp. was the most widely distributed in roots, stems and leaves of Oxytropis trichophora. The phylogenetic analysis of endophytic fungi isolated from Oxytropis trichophora showed that they were divided into two groups according to their genetic relationship. The result indicated there was an obviously difference in quantity, species and population distribution of endophytic fungi between different parts of Oxytropis trichophora. Stem was the most vulnerable to infection and colonization by endophytic fungi, and the diversity was the maximum in stem of Oxytropis trichophora.

Key words: Oxytropis trichophora, endophytic fungi, isolation and identification, population distribution, phylogenetic analysis

CLC Number:

S856.9

SUN Lu, REN Zhen-hui, SONG Run-jie, QUAN Hai-yun, JIAO Yun-jie, HUANG Zhu-tao, WANG Jing-long, ZHAO Bao-yu, LU Hao. Isolation, Identification and Phylogenetic Analysis of Fungal Endophyte in Oxytropis trichophora[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 2025-2035.

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Isolation, Identification and Phylogenetic Analysis of Fungal Endophyte in Oxytropis trichophora

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