麦洼牦牛GH、GHR、GHSR基因的SNPs检测及其与体尺性状的关联分析

doi:10.11843/j.issn.0366-6964.2017.04.003

Abstract

Abstract:

The aims of this study were to determine the genetic diversity of the GH, GHR and GHSR genes in Maiwa yak, to reveal the association between different genotypes and growth traits, to provide the theoretical foundation for the expression regulation of the candidate gene in yak and to look for the molecular marker that can be used for the assistant selection for genetic breeding. The DNA pool technology, PCR-RFLP and the direct sequencing were adopted to study the genetic polymorphism of the GH, GHR and GHSR genes in Maiwa yak and to analyze the association between the polymorphism of the candidate genes and the growth traits, such as withers height, body length, chest circumference, cannon circumference and body weight. The results showed that: 1)The GH, GHR and GHSR genes of the Maiwa yak were all with the polymorphism, among which the GH gene had 2 SNP sites(A757G and T949C). Three mutation sites are found on the GHR gene(T2416C, T3490C and A7500G). T1387C and T3006C mutations existed on the GHSR gene. 2)The fitness test indicated that the A7500G of the GHR gene in the pink lip group and the T3006C of the GHSR gene in the pure black group all deviated from the Hardy-Weinberg equilibrium while the other sites all conformed to the Hardy-Weinberg equilibrium. 3)The different significance test indicated that the T2416C, T3490C and A7500G of the GHR gene were significantly related to the cannon circumference of the Maiwa yak (P<0.01), while the T1387C of the GHSR gene was significantly related to body weight (P<0.05). The results indicate that the GH, GHR and GHSR genes of the Maiwa yak are all with the genetic polymorphism, and it is deduced that the T2416C, T3490C and A7500G of the GHR gene as well as the T1387C of the GHSR gene may be the major effect genes influencing the cannon circumference and body weight of the Maiwa yak or the loci may be closely linked to the major effect genes and they can be regarded as the genetic markers for the assistant selection.

Key words: Maiwa yak, GH gene, GHR gene, GHSR gene, polymorphism, association analysis

CLC Number:

S823.85
Q754

HAI Ting, CHAI Zhi-xin, ZHONG Jin-cheng. Identification of SNPs in GH, GHR and GHSR Genes and Their Association with Body Measurement Traits in Maiwa Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 605-617.

References

[1] 宋桃伟, 罗卫星, 蔡惠芬, 等. 山羊GHSR基因多态性及其与体重和体尺性状的相关性研究[J]. 畜牧兽医学报, 2013, 44(11):1717-1723.
SONG T W, LUO W X, CAI H F,et al. Polymorphism of GHSR gene and its relationship with body weight and body size traits in goat[J]. Acta Veterinaria et Zootechnica Sinica, 2013, 44(11):1717-1723.(in Chinese)
[2] SUN Y X, BUTTE N F, GARCIA J M,et al. Characterization of adult ghrelin and ghrelin receptor knockout mice under positive and negative energy balance[J]. Endocrinology, 2008, 149(2):843-850.
[3] NIE Q, FANG M, XIE L, et al. Molecular characterization of the ghrelin and ghrelin receptor genes and effects on fat deposition in chicken and duck[J]. J Biomed Biotechnol, 2009, 2009:567120.
[4] YAO J B, AGGREYS E, ZADWORNY D, et a1. Sequence variation in the bovine growth hormone gene characterized by SSCP analysis and their association with milk production traits in Holsteins[J]. Genetics,1996,144(4):1809-1816.
[5] 李军. GH和GHR基因部分序列多态性与荷斯坦公牛生长性能、屠宰性能及肉品质的相关性研究[D].兰州:甘肃农业大学, 2012.
LI J. Relationships of single nucleotide polymorphisms in GH and GHR to growth performance, carcass quality and meat quality of holstein bulls[D].Lanzhou:Gansu Agricultural University,2012. (in Chinese)
[6] 牛志刚,史洪才,刘明军,等.新疆褐牛GH基因第5外显子AluⅠ位点多态性与早期生长性状的相关性[J].南方农业学报, 2012, 43(5):688-691.
NIU Z G, SHI H C, LIU M J, et al. Correlation between GH gene polymorphism of the 5th exon Alu Ⅰ site and early growth traits in Xinjiang Brown Cattle[J].Journal of Southern Agriculture, 2012, 43(5):688-691. (in Chinese)
[7] 宋桃伟, 蔡惠芬, 罗卫星, 等. 两个贵州山羊品种GHSR和GHRL基因遗传变异及其与生长性状的关联性[J]. 中国农业科学, 2015, 48(1):140-153.
SONG T W, CAI H F, LUO W X, et al. Association of GHSR and GHRL gene genetic variation with growth traits in two Guizhou goat breeds[J]. Scientia Agricultura Sinica, 2015,48(1):140-153. (in Chinese)
[8] 胡怡菲.秦川牛GH和GHR基因多态性及其与部分体尺性状的关联研究[D].杨凌:西北农林科技大学, 2010.
HU Y F. Study on the polymorphisms in GH and GHR gene and their relationship with partial body measurement traits in Qinchuan cattle[D].Yangling:Northwest A & F University, 2010. (in Chinese)
[9] 白晶晶.牦牛GH基因多态性的PCR-SSCP分析[D].兰州:甘肃农业大学, 2009.
BAI J J. Polymorphism of growth hormone gene by PCR-SSCP in the yak[D]. Lanzhou:Gansu Agricultural University, 2009. (in Chinese)
[10] 张森.牦牛GH基因和GHR基因的多态性及生长发育的性状标记[D].兰州:甘肃农业大学, 2008.
ZHANG S. Polymorphism of GH gene and GHR gene and molecular marker of growth development traits in Yak[D]. Lanzhou:Gansu Agricultural University,2008. (in Chinese)
[11] 刘梅,鞠志花,李秋玲,等. MBL1基因第一内含子与第二外显子多态性与中国荷斯坦牛乳腺炎和乳品质的关联分析[J].中国农业科学, 2010, 43(11):2363-2371.
LIU M, JU Z H, LI Q L, et al. Polymorphisms of MBL1 gene intron 1 and exon 2 and its association with mastitis and milk quality in chinese holstein cattle[J]. Scientia Agricultura Sinica, 2010, 43(11):2363-2371. (in Chinese)
[12] KOPCHIC J K, BELLUS L H, COSCHIGAN L T. Transgenic models of growth hormone action[J]. Annu Rev Nutr, 1999, 19:437-461.
[13] 方梅霞, 李莹, 徐海平,等. GHRL及其受体GHSR基因多态性与鸭生长及屠体性状的关联性[J]. 畜牧兽医学报,2011,42(1):18-24.
FANG M X, LI Y, XU H P, et al. Associations of ghrelin(GHRL) and its receptor(GHSR) genes polymorphisms with duck growth and carass traits[J].Acta Veterinaria et Zootechnica Sinica,2011,42(1):18-24. (in Chinese)
[14] 刘彬, 宋桃伟, 罗卫星,等. GHSR基因多态性与黔北麻羊生长性状关联性分析[J].广东农业科学, 2013, 40(22):161-165.
LIU B, SONG T W, LUO W X, et al. Study on relationship between polymorphism of GHSR gene and growth traits in Qianbei Ma goat[J]. Guangdong Agricultural Sciences, 2013, 40(22):161-165. (in Chinese)
[15] 潘和平. 五个不同地区牦牛部分生产性能候选基因多态性的研究[D]. 兰州:甘肃农业大学, 2008.
PAN H P. Study on polymorphism of candidate gene with some productive performance in yaks from five different regions[D]. Lanzhou:Gansu Agricultural University, 2008. (in Chinese)
[16] 李永红, 常洪, 耿荣庆, 等. 以GH基因多态性探讨中国南北黄牛及牦牛系统[J].畜牧兽医学报,2008, 39(9):1165-1170.
LI Y H, CHANG H, GENG R Q, et al. Study on phylogenesis of chinese southern and northern cattle and yak based on the polymorphisms of GH gene[J]. Acta Veterinaria et Zootechnica Sinica, 2008, 39(9):1165-1170. (in Chinese)
[17] 何桦. 中国荷斯坦牛和牦牛的三个功能基因多态性及其生产性能的相关性分析[D].雅安:四川农业大学,2005.
HE H. The Polymorphism of three functional genes and their relationship with production performances in chinese holstein and yak[D].Ya'an, Sichuan Agricultural University,2005. (in Chinese)
[18] 欧江涛, 钟金城, 赵益新, 等.牦牛生长激素基因的测序和多态性研究[J].黄牛杂志, 2003, 29(2):9-12.
OU J T, ZHONG J C, ZHAO Y X, et al. Sequencing and polymorphism analysison growth hormonegene in Yak[J]. Journal of Yellow Cattle Science, 2003, 29(2):9-12. (in Chinese)
[19] SUN Y X, BUTTE N F, GARCIA J M, et al. Characterization of adult ghrelin and ghrelin receptor knockout mice under positive and negative energy balance[J]. Endocrinology, 2008, 149(2):843-850.
[20] 张娜. 牦牛Ghrelin对其极端生境适应的分子机理[D].兰州:兰州大学, 2016.
ZHANG N. Molecular mechanism of yak (Bos grunniens) ghrelin adaption to its extreme environment[D].Lanzhou:Lanzhou University,2016. (in Chinese)
[21] 赵彦玲, 任子利, 李瑜鑫, 等. 犏牛、牦牛睾丸组织中Ghrelin与GHSR基因mRNA表达水平研究[J]. 广东农业科学, 2016, 43(7):151-157.
ZHAO Y L, REN Z L, LI Y X, et al. Expression level of ghrelin and GHSR mRNA in testis of cattle-yak and yak[J].Guangdong Agricultural Sciences, 2016, 43(7):151-157. (in Chinese)

Identification of SNPs in GH, GHR and GHSR Genes and Their Association with Body Measurement Traits in Maiwa Yak

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Qing, WANG Huihua, LU Zengkui, JIN Meilin, CHU Mingxing, WEI Caihong. Association of the PDGF-D Gene Genetic Variation with Sheep Tail Traits [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 688-700.
[2]	LI Qing, WANG Huihua, LU Zengkui, JIN Meilin, CHU Mingxing, WEI Caihong. Association of the PDGF-D Gene Genetic Variation with Sheep Tail Traits [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 688-700.
[3]	HOU Haobin, LI Haijing, YANG Li, ZHANG Xinhao, SHI Tianpei, WANG Xinyue, ZHAO Zhida, ZHANG Li. Association between NCAPG-DCAF16 Region Polymorphisms and Growth Traits in Dezhou Donkeys [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 302-313.
[4]	HOU Haobin, LI Haijing, YANG Li, ZHANG Xinhao, SHI Tianpei, WANG Xinyue, ZHAO Zhida, ZHANG Li. Association between NCAPG-DCAF16 Region Polymorphisms and Growth Traits in Dezhou Donkeys [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 302-313.
[5]	WANG Jian-wen, LI Lin-ling, WANG Jing-li, MENG Jun, ZENG Ya-qi, YAO Xin-kui, XIN Ya-li. Analysis on ACTN3 Gene Exon Polymorphism of Yili Horse [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1299-1306.
[6]	WANG Jian-wen, LI Lin-ling, WANG Jing-li, MENG Jun, ZENG Ya-qi, YAO Xin-kui, XIN Ya-li. Analysis on ACTN3 Gene Exon Polymorphism of Yili Horse [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1299-1306.
[7]	ZHANG Wen-jian, MA Guang-wei, ZHANG Xiao-fei, CHU Yan-kai, LIU Jing, YANG Hua, YAN Xiao-hong, LI Yu-mao, LI Hui, WANG Ning. Verification and Analysis of Wool Quality Traits-associated CCNY Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1389-1400.
[8]	ZHANG Wen-jian, MA Guang-wei, ZHANG Xiao-fei, CHU Yan-kai, LIU Jing, YANG Hua, YAN Xiao-hong, LI Yu-mao, LI Hui, WANG Ning. Verification and Analysis of Wool Quality Traits-associated CCNY Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1389-1400.
[9]	LIANG Guo-ming, CHEN Feng-jian, ZHAO Qin-tao, LIU Chen-hui, TANG Zhong-lin. Study on SNP Identification, Selective Pressure and Association Analysis with Litter Size for ZP4 Gene in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 793-801.
[10]	LIANG Guo-ming, CHEN Feng-jian, ZHAO Qin-tao, LIU Chen-hui, TANG Zhong-lin. Study on SNP Identification, Selective Pressure and Association Analysis with Litter Size for ZP4 Gene in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 793-801.
[11]	HAI Ting, CHAI Zhi-xin, ZHONG Jin-cheng. Identification of SNPs in GH, GHR and GHSR Genes and Their Association with Body Measurement Traits in Maiwa Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 605-617.
[12]	ZHANG Feng-xia, YANG Ting, AO Hong, TAN Zhen, XING Kai, ZHAI Li-wei, YU Ying, WANG Chu-duan. Novel Genetic Mutations in Porcine SLA-DOB and CD4 Significantly Affect the Routine Blood Traits of Pregnant Landrace Sows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2046-2058.
[13]	ZHANG Feng-xia, YANG Ting, AO Hong, TAN Zhen, XING Kai, ZHAI Li-wei, YU Ying, WANG Chu-duan. Novel Genetic Mutations in Porcine SLA-DOB and CD4 Significantly Affect the Routine Blood Traits of Pregnant Landrace Sows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2046-2058.
[14]	PAN Zhang-yuan，HE Xiao-yun，LIU Qiu-yue，HU Wen-ping，WANG Xiang-yu，GUO Xiao-fei，CAO Xiao-han，DI Ran，CHU Ming-xing. Cloning and Genetic Polymorphism Analysis of mRNA，DNA and Regulatory Region of Ovine GDF9 Gene in 11 Breeds [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1555-1564.
[15]	PAN Zhang-yuan，HE Xiao-yun，LIU Qiu-yue，HU Wen-ping，WANG Xiang-yu，GUO Xiao-fei，CAO Xiao-han，DI Ran，CHU Ming-xing. Cloning and Genetic Polymorphism Analysis of mRNA，DNA and Regulatory Region of Ovine GDF9 Gene in 11 Breeds [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1555-1564.