基于蛋白质组学技术的鹿茸不同部位差异蛋白筛选

doi:10.11843/j.issn.0366-6964.2017.04.004

Abstract

Abstract:

To screen the differentially expressed proteins in different parts of sika deer velvet antler，the parts of sika deer velvet antlers of Lapian, Xuepian and Gupian were selected as experimental material.The differentially expressed proteins were identified by two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-TOF-MS, and analyzed using bioinformatics methods. The result showed that 37 differentially expressed proteins were detected, which were involved in the biological process of multicellular organismal process, detoxification, cellular component organization or biogenesis, cell aggregation, localization, response to stimulus, biological adhesion, developmental process, single-organism process and immune system. There were 18, 5 and 14 up-regulated proteins in the Lapian, Xuepian, Gupian，respectively. We found that EFB1 and CRABP1 might play vital role in the process of velvet antler accelerated growth, and APOA1, FABP4, TTR might play vital role in the process of velvet antler ossification. Taken together, the result provide a basis for further research on the molecular mechanisms involved in the accelerated growth and ossification of deer velvet antler.

Key words: sika deer, velvet antler, different parts, two-dimensional gel electrophoresis, proteome

CLC Number:

S825.2

LIU Hua-miao, JU Yan, ZHANG Ran-ran, XING Xiu-mei. Differentially Expressed Proteins Screening in Different Parts of Sika Deer Velvet Antler by Comparative Proteomics[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 618-626.

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Differentially Expressed Proteins Screening in Different Parts of Sika Deer Velvet Antler by Comparative Proteomics

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