SLA-DOB及CD4基因新突变显著影响长白妊娠母猪的血常规性状

doi:10.11843/j.issn.0366-6964.2017.11.005

Abstract

Abstract:

This study aimed to find single nucleotide polymorphism (SNP) sites and molecular markers affecting the routine blood traits of pregnant sows. Porcine SLA-DOB and CD4 were chosen as candidate genes, blood samples were randomly collected from 358 pregnant Landrace sows and used to detect 19 routine blood indexes, then association analysis was conducted between the polymorphic sites of candidate genes and the routine blood traits.We found that 12 mutations, in which SNP1, SNP2 and SNP3 of SLA-DOB gene as well as Ins9, SNP10 and SNP11 in CD4 gene were all newly discovered mutations. SNP1 was significantly associated with mean platelet volume (MPV) (P<0.01), hematocrit (HCT) and red cell volume distribution width SD (RDW_SD) (P<0.05); SNP2 was extremely significantly associated with MPV(P<0.01); SNP3 was significantly associated with mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) (P<0.05); Ins9 was significantly associated with mean corpuscular volume (MCV) (P<0.05) and MPV (P<0.01); SNP10 and SNP11 were significantly associated with MCV, MCH, MPV (P<0.01) and RDW_SD (P<0.05); meanwhile, SNP10 was significantly associated with MCHC (P<0.05). These novel mutations are potential important polymorphic loci affecting the routine blood traits in pregnant sows. The results suggest that the SLA-DOB and CD4 genes as well as their genetic mutations can be as important candidate genes and key molecular markers affecting immunity of pregnant sows.

CLC Number:

S828.2

ZHANG Feng-xia, YANG Ting, AO Hong, TAN Zhen, XING Kai, ZHAI Li-wei, YU Ying, WANG Chu-duan. Novel Genetic Mutations in Porcine SLA-DOB and CD4 Significantly Affect the Routine Blood Traits of Pregnant Landrace Sows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2046-2058.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.11.005

https://www.xmsyxb.com/EN/Y2017/V48/I11/2046

References

[1] PÉREZ-DE-HEREDIA F,GÓMEZ-MARTÍNEZ S,DÍAZ L E,et al.Influence of sex,age,pubertal maturation and body mass index on circulating white blood cell counts in healthy European adolescents-the HELENA study[J].Eur J Pediatr,2015,174(8):999-1014.
[2] 宋欣,曹随忠,张龙超,等.猪红细胞生成素受体基因突变与血常规性状关联分析[J].畜牧兽医学报,2012,43(11):1697-1702.SONG X,CAO S Z,ZHANG L C,et al.Association of SNPs in erythropoietin receptor gene with blood parameters[J].Acta Veterinaria et Zootechnica Sinica,2012,43(11):1697-1702.(in Chinese)
[3] LI J L,ZARBOCK A,HIDALGO A.Platelets as autonomous drones for hemostatic and immune surveillance[J].J Exp Med,2017,doi:10.1084/jem.20170879.
[4] VIEIRA-DE-ABREU A,CAMPBELL R A,WEYRICH A S,et al.Platelets:versatile effector cells in hemostasis,inflammation,and the immune continuum[J].Semin Immunopathol,2012,34(1):5-31.
[5] GROβJOHANN B,EICHLER P,GREINACHER A,et al.Ceftriaxone causes drug-induced immune thrombocytopenia and hemolytic anemia:characterization of targets on platelets and red blood cells[J].Transfusion,2004,44(7):1033-1040.
[6] 陈芳芳,潘玲,耿照玉,等.MHC分子多态性的起源、演变与抗病机理[J].畜牧兽医学报,2010,41(9):1061-1067.CHEN F F,PAN L,GENG Z Y,et al.Origin,evolvement and resistance mechanism of polymorphism of MHC molecules[J].Acta Veterinaria et Zootechnica Sinica,2010,41(9):1061-1067.(in Chinese)
[7] 胜利,安利峰.MHC及其免疫学意义[J].西北民族学院学报:自然科学版,2000,21(2):54-58.SHENG L,AN L F.Major histocompathbility complex (MHC) and their immunological significance[J].Journal of Northwest Minorities University:Natural Science Edition,2000,21(2):54-58.(in Chinese)
[8] SMITH T P,ROHRER G A,ALEXANDER L J,et al.Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals that the SLA spans the centromere[J].Genome Res,1995,5(3):259-271.
[9] BASKIN L C,POMP D.Rapid communication:mapping of the porcineColipase gene to chromosome 7 using linkage analysis[J].J Anim Sci,1998,76(4):1241-1242.
[10] 郑世军.动物分子免疫学[M].北京:中国农业出版社,2015.ZHENG S J.Veterinary molecular immunology[M].Beijing:China Agriculture Press,2015.(in Chinese)
[11] 周光炎.免疫学原理[M].3版.北京:科学出版社,2013.ZHOU G Y.Principles of immunology[M].3rd ed.Beijing:Science Press,2013.(in Chinese)
[12] HE Y,CHU Q,MA P,et al.Association of bovine CD4 and STAT5b single nucleotide polymorphisms with somatic cell scores and milk production traits in Chinese Holsteins[J].J Dairy Res,2011,78(2):242-249.
[13] 巩元芳,卢昕,王志鹏,等.猪2、7和8号染色体上影响血常规指标的数量性状基因座(QTL)检测[J].生物化学与生物物理进展,2008,35(11):1291-1297.GONG Y F,LU X,WANG Z P,et al.Detection of quantitative trait loci affecting haematological traits on swine chromosomes 2,7 and 8[J].Progress in Biochemistry and Biophysics,2008,35(11):1291-1297.(in Chinese)
[14] ZOU Z Z,REN J,YAN X M,et al.Quantitative trait loci for porcine baseline erythroid traits at three growth ages in a White Duroc×Erhualian F₂,resource population[J].Mamm Genome,2008,19(9):640-646.
[15] YANG S,REN J,YAN X,et al.Quantitative trait loci for porcine white blood cells and platelet-related traits in a White Duroc×Erhualian F₂ resource population[J].Anim Genet,2009,40(3):273-278.
[16] ZHANG Z Y,HONG Y,GAO J,et al.Genome-wide association study reveals constant and specific loci for hematological traits at three time stages in a White Duroc×Erhualian F₂ resource population[J].PLoS One,2013,8(5):e63665.
[17] 刘会杰,王晓铄,王志军,等.大白、长白猪CD4基因启动子多态性及表达差异研究[J].中国畜牧杂志,2014,50(1):10-14.LIU H J,WANG X S,WANG Z J,et al.Study on genetic polymorphism in the promoter of CD4 gene and its expression in Yarkshire and Landrace[J].Chinese Journal of Animal Science,2014,50(1):10-14.(in Chinese)
[18] USMAN T,WANG Y C,LIU C,et al.Novel SNPs in IL-17F and IL-17A genes associated with somatic cell count in Chinese Holstein and Inner-Mongolia Sanhe cattle[J].J Anim Sci Biotechnol,2017,8:5.
[19] FALCONER D S,MACKAY T F C,FRANKHAM R.Introduction to quantitative genetics[M].4th ed.Essex,UK:Pearson Education Limited,1996:280.
[20] 许金根.猪T淋巴细胞亚群的候选基因分析及表达谱研究[D].北京:中国农业大学,2013.XU J G.Analysis of candidate genes and study of expression profile for porcine T lymphocyte subpopulations[D].Beijing:China Agricultural University,2013.(in Chinese)
[21] GUPTA S K.Reproductive immunology[M].Netherlands:Springer,1999:4
[22] 林飞卿,章谷生.细胞免疫学研究进展[M].北京:人民卫生出版社,1980.LIN F Q,ZHANG G S.Progress in cellular immunology[M].Beijing:People's Medical Publishing House,1980.(in Chinese)
[23] 刘宝全.兽医免疫学的进展(二)[J].现代化农业,1988(6):19-20.LIU B Q.Progress in veterinary immunology (Ⅱ)[J].Modernizing Agriculture,1988(6):19-20.(in Chinese)
[24] 张孝全,张跃明,贾珉,等.成年母猪的淋巴细胞转化试验[J].中国兽医科技,1982(9):21-23,68.ZHANG X Q,ZHANG Y M,JIA M,et al.Lymphocyte transformation test of adult sows[J].Chinese Veterinary Science,1982(9):21-23,68.(in Chinese)
[25] 郭峰.红细胞天然免疫与获得性免疫[J].自然杂志,2004,26(4):194-199.GUO F.Red cell innate immunity and acquired immunity[J].Chinese Journal of Nature,2004,26(4):194-199.(in Chinese)
[26] 郭峰.红细胞免疫的研究和意义[J].自然杂志,2002,24(5):268-273.GUO F.Red cell immunology research and significance[J].Chinese Journal of Nature,2002,24(5):268-273.(in Chinese)
[27] SIEGEL I,LIU T L,GLEICHER N.The red-cell immune system[J].Lancet,1981,318(8246):556-559.
[28] 郭峰,张乐之,蔡志扬,等.红细胞调控白细胞免疫功能新的自然实验研究体系[J].解放军医学杂志,2006,31(2):92-94.GUO F,ZHANG L Z,CAI Z Y,et al.A study of modulation of white blood cell immune function by erythrocyte with a new system of immunological experimental system[J].Medical Journal of Chinese People's Liberation Army,2006,31(2):92-94.(in Chinese)
[29] CHEN Z,TANG H,QAYYUM R,et al.Genome-wide association analysis of red blood cell traits in African Americans:the COGENT Network[J].Hum Mol Genet,2013,22(12):2529-2538.
[30] 高淑芳,李培成.血小板免疫介质分泌及与免疫病理关系研究进展[J].国际检验医学杂志,2012,33(5):560-562.GAO S F,LI P C.Platelet immune media secretion and the research progress of its relationship with immune pathological[J].International Journal of Laboratory Medicine,2012,33(5):560-562.(in Chinese)
[31] TAJUDDIN S M,SCHICK U M,EICHER J D,et al.Large-scale exome-wide association analysis identifies loci for white blood cell traits and pleiotropy with immune-mediated diseases[J].Am J Hum Genet,2016,99(1):22-39.

Novel Genetic Mutations in Porcine SLA-DOB and CD4 Significantly Affect the Routine Blood Traits of Pregnant Landrace Sows

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	NIU Naiqi, ZHAO Runze, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 79-86.
[2]	ZHU Xueli, ZHANG Longchao, WANG Lixian, PU Lei, LIU Xin. Association Analysis of AQP9 and RPS10 Gene Polymorphisms with Backfat Thickness in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 87-98.
[3]	YANG Kai, LU Zhuoda, HE Jian, ZHANG Ruiqi, WANG Suqing, LI Kebiao, ZHAO Yunxiang, ZHU Xiaoping, GUO Jinbiao. The Population Effect of Commercial Pigs on the Accuracy of Genomic Selection for Carcass Traits in Duroc Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4943-4951.
[4]	XU Tingting, QI Fenfang, HUANG Shihui, NIU Xi, LI Sheng, RAN Xueqin, WANG Jiafu, XIE Jian. Study of the Polymorphisms of Structural Variation and the Expression of MAP3K4 gene in Xiang Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5046-5055.
[5]	HU Ziping, WANG Ligang, ZONG Wencheng, HOU Renda, SU Yanfang, NIU Naiqi, WANG Lixian, WANG Yuan, ZHNAG Longchao. Genetic Structure Analysis of Jianbai Xiang Pig Population Based on Genomic SNP and ROH [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4117-4125.
[6]	JI Zhengyu, NI Mengru, ZHANG Zhaobo, ZHAO Gan, HUANG Zan, LI Pinghua, HUANG Ruihua, HOU Liming. Research on Adipogenic Capacity and Gene Expression Pattern of in Vitro FAPs Cells Derived from Longissimus Dorsi Muscle of Suhuai Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4126-4142.
[7]	BI Huan, QIN Hai, YUAN Wei, ZHANG Yudan, ZHANG Yiyu, CHEN Wei. Effect of Knocking down TYRP1 Gene on Melanin Production in the Xiang Pig Epidermal Melanocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4143-4153.
[8]	WANG Hui, FENG Baoliang, WU Dan, XIANG Guangming, WANG Nan, MU Yulian, LI Kui, LIU Zhiguo. Research Progress of CD163 Gene and Disease-Resistant Breeding on Porcine Reproductive and Respiratory Syndrome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3127-3138.
[9]	ZHANG Wanfeng, ZHAO Tianzhi, LI Jiao, YOU Ziwei, YANG Yang, CAI Chunbo, GAO Pengfei, CAO Guoqing, GUO Xiaohong, LI Bugao. Study on NR2F2 Gene Regulating Proliferation and Apoptosis of Porcine PK15 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3242-3251.
[10]	LU Chang, DONG Lei, ZHANG Wanfeng, GAO Pengfei, GUO Xiaohong, CAI Chunbo, CAO Guoqing, LI Bugao. Identification and Screening of Single Nucleotide Polymorphism Loci in Jinfen White Pigs Based on Whole Genome Resequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2761-2771.
[11]	YANG Qing, GONG Jing, ZHAO Xueyan, ZHU Xiaodong, GENG Liying, ZHANG Chuansheng, WANG Jiying. Comparison of Array and Resequencing in Pig Genetic Structure Studies [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2772-2782.
[12]	WANG Jinglin, LIU Yangguang, XU Qilong, CHEN Shuo, DENG Zaishuang, CHENG Shiyu, DING Yueyun, ZHENG Xianrui, YIN Zongjun, ZHANG Xiaodong. Genome Structures Variant Analysis and Feature SNPs Screening of Wanyue Black Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2783-2793.
[13]	ZHOU Fuchen, YE Jian, GUO Caijin, ZENG Haiyu, WU Zhenfang, DONG Linsong, CAI Gengyuan. Litter Effect Analysis and Genetic Parameters Estimation of Lean Meat Percentage Trait in Duroc Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2288-2296.
[14]	ZHAO Zhenjian, WANG Shujie, CHEN Dong, JI Xiang, SHEN Qi, YU Yang, CUI Shengdi, WANG Junge, CHEN Ziyang, TANG Guoqing. Population Structure and Genetic Diversity Analysis of Neijiang Pigs Based on Low-coverage Whole Genome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2297-2307.
[15]	TAO Xuan, YANG Xuemei, LIANG Yan, LIU Yihui, WANG Yong, KONG Fanjing, LEI Yunfeng, YANG Yuekui, WANG Yan, AN Rui, YANG Kun, Lü Xuebin, HE Zhiping, GU Yiren. Analysis of Genetic Structure of Conservation Population in Yacha Pig Based on SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2308-2319.