高脂饲料对生长育肥猪肉品质和骨骼肌蛋白质组的影响

doi:10.11843/j.issn.0366-6964.2016.10.014

Abstract

Abstract:

The present study was conducted to investigate the effect of high-fat (HF) diet on meat quality traits and skeletal muscle proteome of growing-finishing pigs.Twenty growing-finishing pigs with an average initial BW of (73.28±0.57) kg were randomly allocated to control group (control diet，supplemented with 10.0% cornstarch) and treatment group (high-fat diet，supplemented with 10.0% lard) to study the effect of dietary fat content on growth performance，meat quality traits and skeletal muscle proteome of growing-finishing pigs.There were 10 replicates in each treatment group with 1 pig per replicate.The experiment lasted 42 d.The results showed that pigs fed the HF diet had a greater average daily gain (P<0.05)，but a lower ratio of feed to gain than pigs fed the control diet (P<0.01).The pH_{45 min}，pH_{24 h}，intramuscular fat content，lightness，redness，yellowness，cooking loss，drip loss and shear force in skeletal muscle of pigs were not affected by dietary fat content (P>0.05).Proteomic analysis revealed that pigs fed the HF diet had greater expression levels of calreticulin，myosin heavy chain，capping protein，pyruvate kinase，enolase，L-lactate dehydrogenase，triosephosphate isomerase，adenylate kinase，apolipoprotein A and B，fatty acid binding protein and heat shock protein 27 and 70 ku，but lower expression levels of myosin light chain and phosphoglucomutase than pigs fed the control diet.In conclusion，the HF feeding could improve the growth performance，and upregulate the expression levels of proteins related to lipid metabolism，glucose and energy metabolism in skeletal muscle of growing-finishing pigs.

Key words: high-fat diet, meat quality, proteome, pigs

CLC Number:

S828
S816.71

XIONG Huo，CAI Xin，LIU Jing-bo，CHEN Liang，ZHANG Hong-fu. Effects of High-fat Diet on Meat Quality Traits and Skeletal Muscle Proteome of Growing-finishing Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2052-2059.

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Effects of High-fat Diet on Meat Quality Traits and Skeletal Muscle Proteome of Growing-finishing Pigs

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