TLR家族基因在产蛋高峰期和停产期鸭肠道中的表达研究

doi:doi: 10.11843/j.issn.0366-6964.2016.10.009

Abstract

Abstract:

This study aimed to investigate expression of toll-like receptors in different segments of intestinal tract in duck at the peak and end of lay.Twenty laying ducks at the peak of lay were randomly assigned to 2 groups.One group ducks were given a restricted feed for 7 days，and then provided with feed ad libitum for 10 days.Another group ducks were provided with feed ad libitum for 17 days.Expression of TLR 1-1，TLR1-2，TLR2-1，TLR2-2，TLR3，TLR4，TLR5，TLR7，TLR15 and TLR21 were analyzed in duck duodenum，jejunum，ileum and caecum by RT-PCR and qRT-PCR methods.The results showed that 10 TLRs genes were all expressed in duodenum，jejunum，ileum and caecum.Except for TLR1-2，TLR2-2，TLR4 and TLR21，the other TLRs genes mRNA expression was significantly different among different segments of duck intestinal tract (P<0.05).Of these，TLR3，TLR5 and TLR7 mRNA expression were higher in duodenum than in the other tissues，while TLR1-1，TLR2-1 and TLR15 mRNA expression were higher in caecum than in the other tissues.Except for TLR4 and TLR7，the other TLRs genes mRNA expression was higher in ducks at the peak of lay than that in ducks at the end of lay (P<0.05).In conclusion，a TLR-mediated innate immune response mechanism exists in the laying duck intestinal tract，and there was stronger ability to recognize pathogens in duck intestinal tract at the peak of lay than that at the end of lay.

Key words: laying duck, toll-like receptors (TLRs), intestinal tract, peak of lay, end of lay

CLC Number:

S834
S813.3

GUO Jia-jia，DAI Zi-chun，LU Chen，ZHANG Tian，SHI Zhen-dan，ZHAO Wei，MA Wei-ming，YING Shi-jia. Expression of Toll-like Receptors in Duck Intestinal Tract at the Peak and End of Lay[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2012-2019.

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Expression of Toll-like Receptors in Duck Intestinal Tract at the Peak and End of Lay

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