成熟前后水牛卵母细胞蛋白质组的初步研究

doi:10.11843/j.issn.0366-6964.2016.10.008

Abstract

Abstract:

To reveal the molecular mechanism underlying in vitro maturation of the buffalo oocyte and select the proteins associated with the oocyte maturation quality，sodium dodecyl sulfate polyacrylamide gel electrophoresis coupled to reverse-phase liquid chromatography tandem mass spectrometry (RP-LC-MS/MS) proteomics technology were applied to identify the buffalo oocyte proteome at germinal vesicle (GV) stage and metaphase II (MII) stages.In total，647 and 570 proteins (FDR<1%) were identified from GV and MII stage buffalo oocytes，respectively.Among them，414 proteins were common identified，233 and 156 proteins were uniquely identified，respectively.The semi-quantitative information of the identified proteins was obtained using spectral counting method.Nine high abundance proteins were identified from GV and MII stage buffalo oocytes，eight of which were common proteins，including MVP，GATM3，PADI6，PRDX2，NE2，DYNLL1，AKR1B1 and LOC786101.HSP90α and GSTP1 were unique high abundance proteins identified in GV and MII stage buffalo oocytes，respectively.It indicated that they might be closely related to buffalo oocyte maturation.Results of GO (Gene Ontology) and KEGG analysis showed that these common identified proteins involved in metabolism pathways，such as pyruvate metabolism，glycolysis，tricarboxylic acid cycle (TCA)，and pentose phosphate pathway，etc，which suggested that high metabolism level was needed to complete meiotic maturation in buffalo oocytes.Proteins involved in oxidative phosphorylation，ribosome，and proteasome pathway were uniquely identified in GV stage oocytes，indicating that high level of oxidative phosphorylation and protein synthesis might exist in GV stage oocyte，and the proteasome pathway might be essential for the maturation progression of buffalo oocytes.Proteins involved in DNA replication，amino sugar and nucleotide sugar metabolism pathway were uniquely identified in MII stage oocytes，demonstrating that the oocytes might need to prepare genetic material for the fertilization and early cleavage divisions.Overall，above results revealed the protein expression profile of buffalo oocytes before and after maturation，they are useful to understand the molecular mechanisms underlying buffalo oocyte maturation.

Key words: buffalo, in vitro maturation, oocyte, proteome

CLC Number:

S823.8+3.3

CHEN Ling-sheng，DAI Xiao-li，XU Yong-ru，LUO Chan，LU Feng-hua，JIANG Jian-rong，SHI De-shun，XU Ping，LI Xiang-ping. Proteomics Analysis of Immature and Mature Buffalo Oocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2003-2011.

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Proteomics Analysis of Immature and Mature Buffalo Oocytes

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