狐狸MC1R基因编码区c.40A>C和c.41C>T相邻变异研究

doi:doi: 10.11843/j.issn.0366-6964.2016.10.010

Abstract

Abstract:

In order to detect the relationship between polymorphism of MC1R gene and coat color in fox，a total of 163 skin samples of 12 coat color foxes were collected.The nucleotide sequence (1 054 bp) of fox MC1R gene were obtained by the method of PCR and direct sequencing，and the polymorphism were analyzed.The population genetics were analyzed using PopGen32 and SHEsis softwares.The effect of mutations on the function of MC1R gene was evaluated using PANTHER software.The relationships between the variable sites and coat color were analyzed by the statistical methods of SPSS bivarate correlation analysis.Two adjacent missense mutations (c.40A>C and c.41C>T) were found in the coding region of fox MC1R gene，which resulted in codon change of p.Thr14Ile or p.Pro14Leu.When 40 site was A，it led to the substitution between threonine (Thr) and isoleucine (Ile).When 40 site was C，it led to the substitution between proline (Pro) and leucine (Leu).All the genotypes of two coat color foxes belonging to Alopex were TT.However，the genotypes of most coat color foxes belonging to Vulpes were CC.It was supposed that 41 site was important in distinguishing Alopex and Vulpes.The in silico functional analysis showed that the amino acid substitution at p.Pro14Leu had significant impact on the function of MC1R.The statistical analysis showed the polymorphism of 41 site had significant low correlation with the fox coat color.The results indicate that SNP c.41C>T in the coding region of the MC1R gene is probably associated with the coat color in fox. In order to detect the relationship between polymorphism of MC1R gene and coat color in fox，a total of 163 skin samples of 12 coat color foxes were collected.The nucleotide sequence (1 054 bp) of fox MC1R gene were obtained by the method of PCR and direct sequencing，and the polymorphism were analyzed.The population genetics were analyzed using PopGen32 and SHEsis softwares.The effect of mutations on the function of MC1R gene was evaluated using PANTHER software.The relationships between the variable sites and coat color were analyzed by the statistical methods of SPSS bivarate correlation analysis.Two adjacent missense mutations (c.40A>C and c.41C>T) were found in the coding region of fox MC1R gene，which resulted in codon change of p.Thr14Ile or p.Pro14Leu.When 40 site was A，it led to the substitution between threonine (Thr) and isoleucine (Ile).When 40 site was C，it led to the substitution between proline (Pro) and leucine (Leu).All the genotypes of two coat color foxes belonging to Alopex were TT.However，the genotypes of most coat color foxes belonging to Vulpes were CC.It was supposed that 41 site was important in distinguishing Alopex and Vulpes.The in silico functional analysis showed that the amino acid substitution at p.Pro14Leu had significant impact on the function of MC1R.The statistical analysis showed the polymorphism of 41 site had significant low correlation with the fox coat color.The results indicate that SNP c.41C>T in the coding region of the MC1R gene is probably associated with the coat color in fox.

Key words: fox, MC1R gene, 40 and 41 sites, polymorphism, coat color

CLC Number:

XU Gui-li，ZHANG Wen-xiang，DUAN Ling-xin，GONG Yuan-fang，GE Mu-xiang，LIU Xie-rong，WANG Shu-peng，GUO Xin-ling，LIU Zheng-zhu. Study on Adjacent Variation of c.40A>C and c.41C>T of Fox MC1R Gene in Different Populations[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2020-2026.

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Study on Adjacent Variation of c.40A>C and c.41C>T of Fox MC1R Gene in Different Populations

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