基于高通量测序的猪鞭虫雌、雄虫差异基因表达分析

doi:10.11843/j.issn.0366-6964.2016.09.021

Abstract

Abstract:

To find transcriptome difference of female and male Trichuris suis，and to enrich pig whipworm transcriptome data，the transcriptomes were analyzed by Illumina Hiseq 2000 high-throughput RNA sequencing.All of the assembled Unigenes were annotated using BLAST search against NR，GO，COG and KEGG databases.The differentially expressed genes were enriched in gene ontology and KEGG pathway.The results showed that 21 026 and 28 886 unigenes were got from female and male T.suis transcriptomes which were assembled from 59 657 854 and 48 414 184 clean reads respectively.The annotated Unigenes of female T.suis to NR，GO，COG and KEGG databases were 11 700，2 287，1 650 and 9 690 respectively，while the data of male T.suis were 12 902，2 414，1 791 and 10 377.There were 4 320 differentially expressed genes found in female and male T.suis，and more than 84.90% of the information were derived from pig whipworm in NR database.Take female T.suis for reference，3 496 genes were up-regulated and 824 genes were down-regulated in male T.suis.There were 906 differentially expressed genes enriched in KEGG pathways，and 7 of them were significantly enriched.Many important genes associated with reproduction including Major sperm protein，Histone H2A/H2B and Vitellogenin，and genes related to pathogenisis，immunity and inflammatory regulation such as protease，Serpin and Cystatin were found in transcriptome of T.suis，in addition to these，there were many genes of unknown functions.This study revealed the number of differentially expressed genes in female and male T.suis，and obtained the function，classification and metabolic pathways of the differentially expressed genes，which enriched T.suis transcriptome information，and provide a solid basis for the study on T.suis-host interactions and developing anti-whipworm drugs.

Key words: Trichuris suis, transcriptome, gene annotation, differentially expressed genes

CLC Number:

S852.73

ZHOU Zuo-yong，SHAO Ao-jun，XU Ting-ting，CHEN Jia-lei，HU Shi-jun. Differential Expression Analysis of Female and Male Trichuris suis by a High-throughput Transcriptome Sequencing Method[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1914-1923.

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Differential Expression Analysis of Female and Male Trichuris suis by a High-throughput Transcriptome Sequencing Method

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