热应激状态下牛血清生化指标、miRNA表达变化及其相关性分析

doi:10.11843/j.issn.0366-6964.2016.09.012

Abstract

Abstract:

The aim of the present study was to assess the changes of miRNA expression levels and serum biochemical indexes of cattle in heat stress.Twenty Red Angus bulls with similar body condition and consistent feeding and management selected from Chongqing Improved Beef Cattle Farm were used in the experiment.Blood samples were collected from each bull in heat stress and non heat-stress to analyze miRNA expression levels and serum biochemical indexes.The results showed that the serum concentrations of Cl-(P<0.05) and malondialdehyde (MDA) (P<0.01) and the activities of lactate dehydrogenase (LDH) (P<0.05) were significantly increased in heat stress in comparison with control.Whereas，the activities of total antioxidation (T-AOC) (P<0.05)，glutathione peroxidase (GSH-Px) (P<0.05) and superoxide dismutase (SOD) (P<0.01) as well as the contents of IgA (P<0.05)，IgG (P<0.05) and interleukin-2 (IL-2) (P<0.01) were remarkably decreased.Meanwhile，the expression levels of miR-181a (P<0.05) and miR-486 (P<0.05) were significantly downregulated and positively correlated with the contents of IgA (P<0.05)，IL-2 (P<0.05) and IgG (P<0.01)，respectively，but that of miR-1246 were significantly upregulated (P<0.01) and positively correlated with serum contents of MDA (P<0.05).In conclusion，the current results suggested that miR-1246，miR-181a，miR-486 might be involved in regulating the immune response and antioxidation process of cattle in heat stress，and could be as a molecular indicator of anti-heat stress.

Key words: heat stress, miRNA, serum biochemical index, correlation

CLC Number:

S823.2

HU Yu，CAI Ming-cheng，WANG Ling，TAN Lin，BI Wu，ZHANG Jia-hui，ZUO Fu-yuan. The Serum Biochemical Indexes and miRNA Expression in Cattle under Heat Stress and Their Correlation Analysis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1840-1847.

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The Serum Biochemical Indexes and miRNA Expression in Cattle under Heat Stress and Their Correlation Analysis

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