2个印记的基因间lncRNAs位于牛Dlk1-Dio3印记区域

doi:10.11843/j.issn.0366-6964.2016.09.013

Abstract

Abstract:

The objective of this study was to find novel imprinted long noncoding RNAs (lncRNAs) in cattle Dlk1-Dio3 domain.Transcripts mapping to this region between Meg8 and Meg9 were under a systematic search and 2 novel lncRNAs were identified，which were named as Linc24062 and Linc24063 according to annotated bibliography of genecode.The expression patterns of Linc24062 and Linc24063 in adult cattle tissues were characterized using reverse transcription polymerase chain reaction (RT-PCR).The results indicated that Linc24062 and Linc24063 were expressed in all 8 tested tissues，including heart，liver，spleen，lung，kidney，subcutaneous fat，skeletal muscle and brain.The imprinting status of Linc24062 and Linc24063 was assessed based on a polymorphism-based sequencing approach.The results showed that Linc24062 and Linc24063 exhibited monoallelic expression in all examined cattle tissues by comparing sequence chromatograms between genomic DNA and cDNA levels at single nucleotide polymorphism (SNP) site，suggesting that Linc24062 and Linc24063 were imprinted in cattle.

Key words: genomic imprinting, intergenic lncRNAs, Dlk1-Dio3 domain, SNP, cattle

CLC Number:

YANG Wen-zhi，ZHANG Ming-yue，WANG Guan-nan，ZHAO Yu-peng，ZHANG Wei-wei，LI Shi-jie. Two Imprinted Long Non-coding RNAs Located in Cattle Dlk1-Dio3 Domain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1848-1852.

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Two Imprinted Long Non-coding RNAs Located in Cattle Dlk1-Dio3 Domain

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