RNA-seq转录组测序分析不同季节对槟榔江水牛精液品质的影响

doi:10.11843/j.issn.0366-6964.2016.07.009

Abstract

Abstract:

To determine the key candidate genes related to semen quality in different seasons，the mRNA profiling of Chinese Betelnut-jiang buffalo were analyzed by RNA-seq.Based on similarities in growth status，5 Betelnut-jiang buffalo bulls were selected and their semen quality were tested (including ejaculate volume，fresh sperm motility，sperm density and abnormality rate).According to the semen quality，the samples of summer (treated as SBL group) and spring (as BL group) were selected for RNA-seq.We collected the whole blood in jugular vein of Betelnut-jiang buffalo bulls，extracted the mRNAs and mixed them within group.Then，the mRNA profiling was used to measure the mRNAs in Betelnut-jiang buffalo bulls.Gene expression levels with >1.5 fold change and P value < 0.05 between the BL and SBL groups were further analyzed.Furthermore，GO and KEGG were used to analyze the genes enrichment.Finally，RT-PCR was used to confirm the RNA-seq result.The results indicated 84 differentially expressed genes.Among these，56 genes were up-regulated and 28 genes were down-regulated in SBL group.The GO search revealed that these differential expression genes were mainly related to stress reaction and immune response.And the KEGG selected the TNF signaling pathway and T cell receptor signaling pathway.Neurotrophin receptor alike death domain（NRADD，also named heat shock factor protein 1(HSF1)），localisation of FOS (FOS) and jun proto-oncogene (JUN)，were strongly associated with semen quality in different seasons which could be used as target genes for further study.

Key words: RNA-seq, Betelnut-jiang buffalo, differently expressed genes, semen quality

CLC Number:

S823.8+3

GENG Hong-hong，ZHANG Jing-yang，LI Lian，WANG Gen-lin. Semen Quality Analysis of Betelnut-jiang Buffalo in Different Seasons by RNA-seq[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1373-1380.

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Semen Quality Analysis of Betelnut-jiang Buffalo in Different Seasons by RNA-seq

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