肉用山羊脂代谢相关基因与肌内脂肪含量的相关性分析

doi:10.11843/j.issn.0366-6964.2016.07.005

Abstract

Abstract:

The aim of this study was to determine the mRNA expression of genes related to lipid metabolism in muscle tissue of different goat breeds，and to analyze their association with intramuscular fat (IMF) content，which was predicted to provide basic data for revealing the mechanism underlying IMF formation.Longissimus dorsi，biceps femoris muscle and triceps brachii muscle from 6 healthy castrated Jianzhou Big-eared goats and Chengdu Brown goats after slaughter，respectively，were collected for detecting IMF content，and the real-time PCR was used to detect the mRNA expression of genes related to lipid metabolism，and their association was analyzed.The result showed that，except for PPARG，the other lipid metabolism related genes(including SREBP1c， THRSP， INSIG1，ACACA，SCD1，DGAT1 and DGAT2) were expressed higher in Jianzhou Big-eared goat than in Chengdu Brown goat.Among them，the expression of THRSP，SCD1 and DGAT2 were significantly higher in Jianzhou Big-eared goat than in Chengdu Brown goat in different tissues (P<0.05).Except for ACACA，the expression of other lipid metabolism related genes were the highest in longissimus dorsi of Jianzhou Big-eared goat，with no significant difference between biceps femoris muscle and triceps brachii muscle (P>0.05) (except for PPARG).While the mRNA expression of THRSP and SCD1 were the highest in longissimus dorsi (P<0.05)，the lipid metabolism related genes had no significant differences between biceps femoris muscle and triceps brachii muscle in Chengdu Brown goat (P>0.05).Jianzhou Big-eared goat had higher IMF content than Chengdu Brown goat in all the 3 tissues (P<0.05)，longissimus dorsi had higher IMF content compared with the other tissues.THRSP，DGAT2 and SCD1 showed positive association with IMF in various tissues of Jianzhou Big-eared goat and Chengdu Brown goat.These results indicate that THRSP，SCD1 and DGAT2 may play important role in promoting fat deposition in meat goat，and also provide basic data for revealing the mechanism underlying goat IMF formation.

Key words: meat goat, fat deposition, IMF, association

CLC Number:

S827
S813.3

ZHU Wu-zheng，LIN Ya-qiu，JIANG Ming-feng，WANG Yong，LIAO Hong-hai，LI Qian，ZHU Jiang-jiang. Association Analysis of Intramuscular Fat Content and the Expression of Genes Related to Lipid Metabolism in Meat Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1333-1341.

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Association Analysis of Intramuscular Fat Content and the Expression of Genes Related to Lipid Metabolism in Meat Goat

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