肉用山羊脂代谢相关基因与肌内脂肪含量的相关性分析

doi:10.11843/j.issn.0366-6964.2016.07.005

Abstract

Abstract:

The aim of this study was to determine the mRNA expression of genes related to lipid metabolism in muscle tissue of different goat breeds，and to analyze their association with intramuscular fat (IMF) content，which was predicted to provide basic data for revealing the mechanism underlying IMF formation.Longissimus dorsi，biceps femoris muscle and triceps brachii muscle from 6 healthy castrated Jianzhou Big-eared goats and Chengdu Brown goats after slaughter，respectively，were collected for detecting IMF content，and the real-time PCR was used to detect the mRNA expression of genes related to lipid metabolism，and their association was analyzed.The result showed that，except for PPARG，the other lipid metabolism related genes(including SREBP1c， THRSP， INSIG1，ACACA，SCD1，DGAT1 and DGAT2) were expressed higher in Jianzhou Big-eared goat than in Chengdu Brown goat.Among them，the expression of THRSP，SCD1 and DGAT2 were significantly higher in Jianzhou Big-eared goat than in Chengdu Brown goat in different tissues (P<0.05).Except for ACACA，the expression of other lipid metabolism related genes were the highest in longissimus dorsi of Jianzhou Big-eared goat，with no significant difference between biceps femoris muscle and triceps brachii muscle (P>0.05) (except for PPARG).While the mRNA expression of THRSP and SCD1 were the highest in longissimus dorsi (P<0.05)，the lipid metabolism related genes had no significant differences between biceps femoris muscle and triceps brachii muscle in Chengdu Brown goat (P>0.05).Jianzhou Big-eared goat had higher IMF content than Chengdu Brown goat in all the 3 tissues (P<0.05)，longissimus dorsi had higher IMF content compared with the other tissues.THRSP，DGAT2 and SCD1 showed positive association with IMF in various tissues of Jianzhou Big-eared goat and Chengdu Brown goat.These results indicate that THRSP，SCD1 and DGAT2 may play important role in promoting fat deposition in meat goat，and also provide basic data for revealing the mechanism underlying goat IMF formation.

CLC Number:

S827
S813.3

ZHU Wu-zheng，LIN Ya-qiu，JIANG Ming-feng，WANG Yong，LIAO Hong-hai，LI Qian，ZHU Jiang-jiang. Association Analysis of Intramuscular Fat Content and the Expression of Genes Related to Lipid Metabolism in Meat Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1333-1341.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.07.005

https://www.xmsyxb.com/EN/Y2016/V47/I7/1333

References

［1］TALPUR F N，BHANGER M I，SHERAZI S T.Intramuscular fatty acid profile of longissimus dorsi and semitendinosus muscle from Pateri goats fed under traditional feeding system of Sindh，Pakistan［J］.Meat Sci，2008，80(3)：819-822.
［2］WCBB E C，CASCY N H，SIMCLA L.Goat meat quality［J］.Small Rumin Res， 2005，60(1-2)：153-166.
［3］CASEY N H.Goat meat in human nutrition［C］// International conference on goats，1992，2：582-589.
［4］WOOD J D，ENSER M，FISHER A V，et al.Fat deposition，fatty acid composition and meat quality：A review［J］.Meat Sci，2008，78(4)：343-358.
［5］SHI H B，LUO J，YAO D W，et al.Peroxisome proliferator-activated receptor-γ stimulates the synthesis of monounsaturated fatty acids in dairy goat mammary epithelial cells via the control of stearoyl-coenzyme A desaturase［J］.J Dairy Sci，2013，97(12)：7844-7853.
［6］SHI H B，LUO J，ZHU J J，et al.PPARγ regulates genes involved in triacylglycerol synthesis and secretion in mammary gland epithelial cells of dairy goats［J］.PPAR Res，2013：310948.
［7］HARVATINE K J，BAUMAN D E.SREBP1 and thyroid hormone responsive spot 14 (S14) are involved in the regulation of bovine mammary lipid synthesis during diet-induced milk fat depression and treatment with CLA［J］.J Nutr，2006，136(10)：2468-2474.
［8］EBERLÉ D，HEGARTY B，BOSSARD P，et al.SREBP transcription factors：master regulators of lipid homeostasis［J］.Biochimie，2004，86(11)：839-848.
［9］KANG Y，HENGBO S，JUN L，et al.PPARG modulated lipid accumulation in dairy GMEC via regulation of ADRP gene［J］.J Cell Biochem，2015，116(1)：192-201.
［10］许会芬，罗军，李芳，等.山羊SREBP-1基因的超表达对脂肪酸代谢相关基因表达的影响［J］.生物工程学报，2012，28(11)：1306-1316.
XU H F，LUO J，LI F，et al.Effects of SREBP-1 over-expression on fatty acid metabolism related genes expression in goats［J］.Chinese Journal of Biotechnology，2012，28(11)：1306-1316.(in Chinese)
［11］BIONAZ M，LOOR J J.Gene networks driving bovine milk fat synthesis during the lactation cycle［J］.BMC Genomics，2008，9：366.
［12］SATO R.Sterol metabolism and SREBP activation［J］.Arch Biochem Biophys，2010，501(2)：177-181.
［13］杜静，张连峰，秦川.INSIG-SCAP-SREBP对脂质合成调控的研究进展［J］.中国比较医学杂志，2006，16(12)：772-775.
DU J，ZHANG L F，QIN C.The role of INSIG-SCAP-SREBP in the regulation of lipid metabolism.A review［J］.Chinese Journal of Comparative Medicine，2006，16(12)：772-775.(in Chinese)
［14］KIM Y S，KOLATTUKUDY P E.Malonyl-CoA decarboxylase from the mammary gland of lactating rat.Purification，properties and subcellular localization［J］.Biochim Biophys Acta，1978，531(2)：187-196.
［15］MORRIS C A，CULLEN N G，GLASS B C，et al.Fatty acid synthase effects on bovine adipose fat and milk fat［J］.Mamm Genome，2007，18(1)：64-74.
［16］石恒波，罗军，朱越，等.奶山羊 SCD 基因 CDS 区的克隆、序列分析及过表达［J］.中国农业科学，2012，45(24)：5091-5101.
SHI H B，LUO J，ZHU Y，et al.Cloning，sequence analysis and over-expression of SCD gene of dairy goat［J］.Scientia Agricultura Sinica，2012，45(24)：5091-5101.(in Chinese)
［17］NTAMBI J M.Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol［J］.J Lipid Res，1999，40(9)：1549-1558.
［18］HARRIS C A，HAAS J T，STREEPER R S，et al.DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes［J］.J Lipid Res，2011，52(4)：657-667.
［19］ZHU W Z，LIN Y Q，LIAO H H，et al.Selection of reference genes for gene expression studies related to intramuscular fat deposition in Capra hircus skeletal muscle［J］.PLoS One，2015，10(3)：e0121280.
［20］SHARMA U，BANERJEE P，JOSHI J，et al.Ubiquitous expression of genes in tissues of goat (Capra hircus) using RNA-seq［J］.Inter J Anim Vet Adv，2012，4(4)：292-302.
［21］LI L，JIANG J，WANG L，et al.Expression patterns of peroxisome proliferator-activated receptor gamma 1 versus gamma 2，and their association with intramuscular fat in goat tissues［J］.Gene，2013，528(2)：195-200.
［22］李健.西杂牛PPARγ2、PGC-1α、MEF2C基因表达量及其与肌内脂肪含量、嫩度的相关分析［D］.雅安：四川农业大学，2010.
LI J.The gene expression of PPARγ2、PGC-1αand MEF2C and their correlation analysis with intramuscular fat content and tenderness in simmental hybridization［D］.Ya’an：Sichuan Agricultrual University，2010.(in Chinese)
［23］HUANG Z G，XIONG L，LIU Z S，et al.The developmental changes and effect on IMF content of H-FABP and PPARgamma mRNA expression in sheep muscle［J］.Acta Genetica Sinica，2006，33(6)：507-514.
［24］GRIINARI J M，CORL B A，LACY S H，et al.Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Delta(9)-desaturase［J］.J Nutr，2000，130(9)：2285-2291.
［25］金世杰，李香子，高青山，等.不同生长阶段延边黄牛肌间脂肪含量与SREBP1和SCD1基因表达相关分析［C］//第九届（2014）中国牛业发展大会，2014：165-175.
JIN S J，LI X Z，GAO Q S，et al.Association analysis of Yanbian bovine intramuscular fat content and the expression of SREBP1 and SCD1 in different growth stages［C］//The Ninth Conference for Chinese Cattle Industry Development，2014：167-175.(in Chinese)
［26］DONNELLY C，OLSEN A M，LEWIS L D，et al.Conjugated linoleic acid (CLA) inhibits expression of the Spot 14 (THRSP) and fatty acid synthase genes and impairs the growth of human breast cancer and liposarcoma cells［J］.Nutr Cancer，2009，61(1)：114-122.
［27］ZHU Q，ANDERSON G W，MUCHA G T，et al.The Spot 14 protein is required for de novo lipid synthesis in the lactating mammary gland［J］.Endocrinology，2005，146(8)：3343-3350.
［28］GUO B，KONGSUWAN K，GREENWOOD P L，et al.A gene expression estimator of intramuscular fat percentage for use in both cattle and sheep［J］. J Anim Sci Biotechnol， 2014，5(1)：35.
［29］KADEGOWDA A K，BIONAZ M，THERING B，et al.Identification of internal control genes for quantitative polymerase chain reaction in mammary tissue of lactating cows receiving lipid supplements［J］.J Dairy Sci，2009，92(5)：2007-2019.
［30］陈利，赵薇，占思远，等.山羊不同组织及不同发育时期骨骼肌内参基因的表达稳定性分析［J］.畜牧兽医学报，2014，45(8)：1228-1236.
CHEN L，ZHAO W，ZHAN S Y，et al.The expression stability analysis of reference genes in the different tissues and skeletal of different development periods in goat［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(8)：1228-1236.(in Chinese)

[1]	LI Li-sha, PENG Yong-dong, ZHENG Xiao-ning, LI Xiang-long. Analysis of the Promoter Activity and Transcriptional Regulatory Elements of Goat PMEL Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 826-835.
[2]	ZHU Jiang-jiang, LIN Ya-qiu, WANG Yong, LI Qian, LIN Sen. Molecular Cloning and Tissue Expression of Goat Chemokine (CC motif) Receptor 2 (CCR2) Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2202-2209.
[3]	WANG Miao，LUO Jun，XU Hui-fen，ZHU Jiang-jiang，HE Qiu-ya，YAO Da-wei，SHI Huai-ping. Effect of INSIG1 Overexpression on the Lipid Synthesis in Goat Mammary Gland Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1806-1816.
[4]	SUN Hong-xin，WANG Hong-na，ZHANG Ying-jie，LIU Yue-qin，CHEN Xiao-yong，DUN Wei-tao. Cloning and Expression of BMPR-IB Gene in Cashmere Goat Fibroblast Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1124-1132.
[5]	LIN Ya-qiu，LIAO Hong-hai，HE Qing-hua，LI Qian，WANG Yong. Cloning and Expression Profiling of FTO Gene of Goat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 888-898.
[6]	DU Chen，LI Jin-quan，CHEN Xiu-juan. Construction of Lentiviral RNAi Vector of PPARγ Gene in Cashmere Goat and Its Effect on Proliferation and Differentiation of Intramuscular Adipocytes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 671-678.
[7]	JIA Wen-chao，LIU Liang-liang，WU Xian-feng，ZHAO Zhao-yan，WANG Ke，WANG Mao，GAO Jing，WANG Li-qiang，CHEN Hong，PAN Chuan-ying，LAN Xian-yong. Molecular Cloning，Bioinformatics Analysis and Methylation Modification of Goat STAT3 Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 457-466.
[8]	LIAO Hong-hai，LIN Ya-qiu，WU Yang-nan，ZHU Wu-zheng，LI Qian，WANG Yong，CHEN Juan. Molecular Cloning，Bioinformatics Analysis and Expression of DLK1 Gene in Goat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 467-476.
[9]	GENG Li-ying，ZHANG Yu，LI Xiang-long，ZHOU Rong-yan，LI Lan-hui，WANG Zhi-gang. Activity and Regulation Region Analysis of Promoter of Goat FOXL2 Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2153-2160.
[10]	ZHANG Tian，LI Xiang-long，ZHOU Rong-yan，LI Lan-hui. Study on Agouti Spliceosome Types in Goat Skin with Different Coat Color [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 1934-1943.
[11]	MIN Ling-jiang，FENG Yan-ni，LI Lan，LI Mei-yu. Associations of MSTN Gene’s Polymorphisms with Some Economic Traits in Goats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1515-1524.
[12]	REN Hang-xing，WANG Gao-fu，LU Jian，LI Jie，JIANG Jing，LIU Liang-jia，ZHOU Peng. The Characteristic Histomorphology of Various Skin Colors and Expression of Genes Involved in Melanogenesis in Goats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1525-1531.
[13]	HUANG Dong-wei，CHU Ming-xing，DI Ran，LIU Qiu-yue，HU Wen-ping，WANG Xiang-yu，PAN Zhang-yuan，GUO Xiao-fei. Study on Expression of KiSS-1 and RFRP Genes Related to Reproductive Seasonality in Goats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 924-931.
[14]	LAN Rong，ZHU Lan，YAO Xin-rong，WANG Peng，SHAO Qing-yong，HONG Qiong-hua. Genome-wide Association Study of Lambing Number in Goat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 549-554.
[15]	LI Yin-xia，ZHANG Jun，QIAN Yong，LI Jing-xin，MENG Chun-hua,WANG Hui-li,ZHONG Sheng，CAO Shao-xian. Kiss-1 Gene Polymorphisms and Its Association with Litter Sizes in Suhuai Goats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 1917-1923.

Association Analysis of Intramuscular Fat Content and the Expression of Genes Related to Lipid Metabolism in Meat Goat

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments