美洲水貂刺鼠信号蛋白基因SNPs检测及其与毛色表型的关联分析

doi:10.11843/j.issn.0366-6964.2016.04.012

Abstract

Abstract:

The aim of this study was to research single nucleotide polymorphisms of agouti signal protein(Agouti)gene and analyze the association of SNPs with coat color phenotype in American mink(Neovison vison).The DNA of blood from 5 mink breeds(Jinzhou black，Jilin white，Silverblue，Coffee and Pearl mink)that possessed significant difference in coat color phenotype were selected as templates，SNP sites were screened by PCR and Sanger double chain termination sequencing technology，and then the relationship between the mutation sites of Agouti gene and coat color phenotype were analyzed in 430 minks.The results showed that the obtained mink Agouti gene was 2 510 bp in length.Total 10 SNPs were screened from 430 individuals of 5 coat color breeds，of which 4 SNPs (g.18G>A，g.159A>G，g.235G>T and g.1189C>T)were located in intron 2 and 6 SNPs(g.252C>T，g.290A>C，g.298G>C，g.340A>G，g.343T>C and g.379T>C)were detected from partial intron 3 of Agouti gene，while no SNPs was discovered in exon 2 and 3.Association analysis of 7 SNPs in Agouti gene with coat color phenotype indicated that all sites were very significantly(P<0.000 1)correlated with coat color phenotype of American mink.In addition，the 5 loci including g.290A>C，g.298G>C，g.340A>G，g.343T>C and g.379T>C might show closely linkaged phenomenon.Results of the present study indicated that Agouti gene might be the candidate gene or linked with the major gene affecting the coat color phenotype of American mink.

Key words: American mink, Agouti gene, coat color phenotype, SNPs, correlation analysis

CLC Number:

S865.2⁺2.2

SONG Xing-chao，XU Chao，LIU Zong-yue，YUE Zhi-gang，CONG Bo，LIU Lin-ling，YANG Fu-he. Single Nucleotide Polymorphisms Detection of Agouti Gene and Its Association with Coat Color Phenotype in American Mink(Neovison vison)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 723-732.

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Single Nucleotide Polymorphisms Detection of Agouti Gene and Its Association with Coat Color Phenotype in American Mink(Neovison vison)

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