培育方式对双胞胎湖羊羔羊肝基因表达的影响

doi:10.11843/j.issn.0366-6964.2016.04.013

Abstract

Abstract:

This experiment was conducted to study the effect of different rearing systems on hepatic gene expression in twin lambs.Twenty-four pairs of Hu male twin lambs were equally divided into 2 groups.Twenty-four subjects were artificially reared(AR) with milk replacer after 7 days colostrum while 24 others were ewe reared(ER) with mother milk as control.All lambs were weaned at day 60 and then fed with starter till day 90.When the lambs reached 60 and 90 days old，three pairs of twin lambs at each time point were slaughter to collect liver samples.Hepatic gene expression were analyzed via microarray and bioinformatics method.We found many differentially expressed genes(DEGs).They were OR1E2，S1PR3，KMO，APOA4，ORM2，HTR4，FADS1 in both 60AR vs 60ER and 90AR vs 90ER，and ACO1，HBA1，MFSD2A，CYSJ，LOC785954，FCN，GRB2，LOC100037663，ORM2，RXRG，LOC100602447，SQLE，HSD17B2，CYP4F2，INHBE，EAAT2 in both 60AR vs 90AR and 60ER vs 90ER，among which 4 unknown genes were identified with the probe ID 14780690，14815720，14845504，14854080.ORM2 was differentially expressed among all the comparisons.Those DEGs involved in the amino acid metabolism，small molecular metabolism，enzyme activities and immune response of GO process and amino acid metabolism，lipid metabolism，hormone biosynthesis and signaling transductions of KEGG pathways.In summary，different rearing systems altered hepatic gene expression.The pathways and functions those DEGs involved in could shed lights on the mechanisms of early cultivation of lambs.

Key words: twin lambs, early weaning, milk replacer, differentially expressed gene, genechip

CLC Number:

S826
S815.4

WANG Hai-chao，ZHANG Nai-feng，CHAI Jian-min，WANG Bo，DIAO Qi-yu. Effect of Different Rearing Systems on Hepatic Gene Expression of Early Weaned Hu Twin Lambs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 733-744.

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Effect of Different Rearing Systems on Hepatic Gene Expression of Early Weaned Hu Twin Lambs

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