鹅乙酰辅酶A酰基转移酶2基因的克隆及其在鹅肥肝形成过程中的表达变化

doi:10.11843/j.issn.0366-6964.2016.04.009

Abstract

Abstract:

To explore the relationship between ACAA2 gene and liver fatty metabolism in goose，35 Landes geese were divided into overfeeding group(15) and control group(20)，the overfeeding group included 3 periods：overfeeding 7，14 and 19 days.The full coding sequence (CDS) of ACAA2 was cloned by RT-PCR and analyzed，its expression levels at different stages of overfeeding were determined in both normal and fatty liver by qRT-PCR in Landes geese.In addition，goose primary hepatocytes were treated with glucose，fatty acids or insulin to determine how its expression was regulated during fatty liver formation.Data indicated that the complete CDS of goose ACAA2 was 1 194 bp and encoded 397 amino acids.The ACAA2 expression level in the livers of the overfed geese was significantly higher (P<0.05) than the control at every stage，and presented a declining trend with the extension of overfeeding time.Moreover，compared to the control，the expression of ACAA2 gene was significantly increased in the goose primary hepatocytes treated with 0.5 mmol•L^-1 oleate，but was decreased in the primary hepatocytes treated with 0.25 mmol•L^-1 palmitate and insulin with different concentrations (P<0.05).The results suggeste that ACAA2 expression is closely related to the development of goose fatty liver，which will provide a basis for further study the role of ACAA2 gene in the formation of goose fatty liver.

Key words: goose, Acetyl-coenzyme A acyltransferase 2, fatty liver, gene cloning

CLC Number:

S835
S813.3

WANG Qian-qian，YANG Biao，XIA Li-li，SUN Xiao-xian，GENG Tuo-yu，GONG Dao-qing. Cloning of Goose Acetyl-Coenzyme A Acyltransferase 2 and Its Expression Pattern in the Development of Fatty Liver[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 700-708.

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Cloning of Goose Acetyl-Coenzyme A Acyltransferase 2 and Its Expression Pattern in the Development of Fatty Liver

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