脂多糖对体外培养猪颗粒细胞增殖、凋亡和雌二醇分泌的影响

doi:10.11843/j.issn.0366-6964.2016.04.008

Abstract

Abstract:

In order to study the effects of Lipopolysaccharide（LPS） on proliferation，apoptosis and estradiol (E₂) secretion of porcine granulosa cells.Primary porcine granulosa cells were cultured in vitro and treated by LPS at different concentrations (0，500，1 000 and 2 000 ng•mL^-1).Then cell proliferation，apoptosis，secretion of E₂ and related gene expressions，FN1，IGF2，IGFBP2，Cyclin D1，Cyclin D2，P27^kip and P450arom were determined individually.The results showed that cell proliferation and survival ratio were significantly promoted at LPS concentrations of 1 000 or 2 000 ng•mL^-1(P<0.05)，on the contrary，cell apoptosis was inhibited.However，gene expression levels of FN1 (P<0.01)，IGF2，IGFBP2 (P<0.05)，Cyclin D1 and Cyclin D2 (P<0.01) were up-regulated at 500 ng•mL^-1 of LPS，P27^kip (P<0.01) which hindered cell cycle was inhibited.P450arom gene expression was decreased，resulting the inhibition of E₂secretion when treated by LPS.These results suggested that granulosa cell proliferation could be promoted by LPS，while cell apoptosis and E₂secretion were inhibited.

Key words: granulosa cells, LPS, cell proliferation, cell apoptosis, gene expression, E₂

CLC Number:

S828
S814.1

GUO Shuang-shuang，LI Hui，SHI Zhen-dan，MA Wei-ming. Effects of Lipopolysaccharide on Proliferation，Apoptosis and Estradiol Secretion of in vitro Cultured Porcine Granulosa Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 693-699.

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Effects of Lipopolysaccharide on Proliferation，Apoptosis and Estradiol Secretion of in vitro Cultured Porcine Granulosa Cells

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