新城疫疫苗毒和低致病性禽流感病毒感染SPF鸡炎症因子的变化

doi:10.11843/j.issn.0366-6964.2015.04.019

Abstract

Abstract:

The aim of the present study was to investigate if the changes of inflammatory cytokines (interleukin 1，interleukin 6，interleukin 8，tumor necrosis factor alpha) and tracheal washing liquid and trachea mucosal secretory immunglobulin A (SIgA) can be used as judgments or warning indicators of respiratory diseases.We established a respiratory diseases model by infecting SPF chickens with Newcastle disease LaSota live vaccine and low pathogenic influenza virus (H9N2) single or mixed.Inflammatory factors of serum and SIgA were measured by double antibody sandwich ELISA method to analyze the inner relationship between the quantity changes and respiratory diseases.The results showed that SPF chickens appeared respiratory symptoms after inoculating LaSota vaccine and H9N2 AIV，content of inflammatory factors and mucosal antibody became significantly higher than those in control group，the changes of five cytokines content induced by two viral infection had obvious differences with time going，inflammation mediums became roughly same while the content in serum were less than in washing liquid.Pathological changes of chicken respiratory tract infection were closely related to the changing process of five inflammatory factors.With the help of detection of inflammatory factors，especially the IL-8，the severity of respiratory tract inflammation could be identified，so the detection of its content would have important reference value for the judgment of respiratory illness and early warning.

Key words: chicken, respiratory diseases, inflammatory factors, diagnosis, prediction

CLC Number:

S852.4

NIU Yu-juan，MA Hai-ying，ZHANG Zhen-dong，Lü Chuan-wei，ZHU Ming-xia，LIU Si-dang. The Changes of Inflammatory Factors in SPF Chicken infected by LaSota live Vaccine and H9N2 Influenza Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 644-649.

References

［1］李长振，饶菁菁，黄永国，等.军团菌肺炎患儿血清铁蛋白水平变化及意义［J］.广东医学，2012，33（11）：1571-1573.
LI C Z，RAO J J，HUANG Y G，et al.The significance of the serum ferritin in Legionella pneumonia in children ［J］．Guangdong Medical Journal，2012，33(11)：1571-1573.（in Chinese）
［2］HIGA F，AKAMINE M，FURUGEN M，et al．Hepatocyte growth factor levels in Legionella pneumonia：a retrospective study ［J］．BMC Infect Dis，2011，11：74.doi：10.1186/1471-2334-11-74.
［3］WANG W Y，LIM J H，LI J D．Synergistic and feedback signaling mechanisms in the regulation of inflammation in respiratory infections ［J］．Cell Mol Immunol，2012，9(2)：131-135．
［4］DOERNBERG S,SCHAAF B,DALHOFF K，et al． Association of macrophage migration inhibitory factor（MIF）polymorphisms with risk of meningitis from Streptococcus pneumoniae ［J］．Cytokine，2011，53(3)：292-294．
［5］ANTTILA M，PELTOLA H.Serum C-reactive protein in the course of Haemophilus influenzae type b meningitis ［J］．J Infect Dis，1992，165(Suppl 1)：S36-S37．
［6］WANG X，JIANG J，CAO Z，et al．Diagnostic performance of multiplex cytokine and chemokine assay for tuberculosis ［J］．Tuberculosis (Edinb)，2012，92(6)：513-520.
［7］WONG R S，WU A，TO K F，et al．Haematological manifestations in patients with severe acute respiratory syndrome：retrospective analysis［J］．BMJ，2003，326（7403）：1358-1362．
［8］张永宏，陈新月，闫惠平，等.SARS患者血清细胞因子临床意义探讨［J］.中华微生物学和免疫学杂志，2004，24(3)：178-180.
ZHANG Y H，CHEN X Y，YAN H P，et al．Discussion on clinical signification of cytokine in severe acute respiratory syndrome［J］．Chinese Journal of Microbiology and Immunology，2004，24(3)：178-180.(in Chinese)
［9］VIEIRA R A，DINIZ E M，CECCON M E.Correlation between inflammatory mediators in the nasopharyngeal secretion and in the serum of children with lower respiratory tract infection caused by respiratory syncytial virus and disease severity ［J］．J Bras Pneumol，2010，36(1)：59-66．
［10］VAN REETH K.Cytokines in the pathogenesis of influenza ［J］.Vet Microbiol，2000，74(1-2)：109-116.
［11］DENG R，LU M，KORTEWEG C，et al.Distinctly different expression of cytokines and chemokines in the lungs of two H5N1 avian influenza patients ［J］. J Pathol，2008，216(3)：328-336.
［12］HAYDEN F G,FRITZ R,LOBO M C，et al.Local and systemic cytokine responses during experimental human infuenza A virus infection ［J］.J Clin Invest，1998，101(3)：643-649.
［13］ARNDT U，WENNEMUTH G，BARTH P，et al.Release of macrophage migration inhibitory factor and CXCL8/interleukin-8 from lung epithelial cells rendered necrotic by influenza A virus infection ［J］.J Virol，2002，76(18)：9298-9306.
［14］闫振贵.不同途径免疫NDV弱毒苗诱导鸡体黏膜和系统免疫反应的研究［D］.泰安：山东农业大学，2011.
YAN Z G.The Study of mucosal and systemic immune responses of chickens induced by different vaccination routes with attenuated NDV vaccine［D］.Taian：Shandong Agricultural University，2011.(in Chinese)
［15］曹殿军，孙建宏，闫丽辉，等.新城疫LaSota、V4疫苗免疫鸡和免疫攻毒鸡的特异性IgA抗体动态变化规律比较［J］.中国预防兽医学报，2001，23(2)：84-86.
CAO D J，SUN J H，YAN L H，et al.Comparison of NDV specific IgA antibodies titre from LaSota and V4 vaccine immunized chickens and F48E9 challenged immunized chickens［J］.Chinese Journal of Preventive Veterinary Medicine，2001，23(2)：84-86.(in Chinese)

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The Changes of Inflammatory Factors in SPF Chicken infected by LaSota live Vaccine and H9N2 Influenza Virus

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