山东地区鸡传染性支气管炎病毒抗原相关性和血清分型

doi:10.11843/j.issn.0366-6964.2015.04.015

Abstract

Abstract:

To clarify the epidemiology of infectious bronchitis virus (IBV) in Shandong province，the antigenic difference among field isolates and vaccine strains was studied.The monovalent antisera against 3 commercial IBV vaccine strains (H120，Ma5 and 4/91) and 18 field isolates were prepared in SPF chickens.The cross viral neutralization (VN) test was performed in SPF chicken embryos to determine the endpoint titers for homologous and heterologous neutralization.The serotype of IBV strains was determined based on antigenic relatedness values (ARV) calculated by the formula R=r1×r2.As a result，vaccine strain 4/91 was belong to serotype I.Vaccine strain H120，Ma5 and two isolates （SDWF0608 and SDLY0701）were grouped in the same serotype.SDZB0808 and the other 15 isolates were grouped in the same serotype.CK/CH/SD09/006 and CK/CH/SD10/001 were in 2 other different serotypes，together with other 3 or 6 IBV isolates，respectively.Moreover，some IBV strains showed one-way neutralizing reaction with others and some IBV strains in the same serotype had different ARVs.The resultes indicate that the antigenicity of IBV strains is very complicated and there are evident antigenic difference between IBV isolates and 3 vaccine strains.

Key words: infectious bronchitis virus, cross neutralization test, antigenic relatedness value, serotype

CLC Number:

S852.659.6

HU Bei-xia，XU Chuan-tian，YANG Shao-hua，HUANG Yan-yan，ZHANG Lin，HUANG Qing-hua，HUO Ya-fei，ZHANG Wei，ZHANG Xiu-mei. Antigenic Relatedness and Serotype of Infectious Bronchitis Virus Isolated in Shandong Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 615-623.

References

［1］MASTERS P S.The molecular biology of coronaviruses［J］.Adv Virus Res，2006，66:193-292.
［2］LI M，MO M L，HUANG B C，et al.Continuous evolution of avian infectious bronchitis virus resulting in different variants co-circulating in Southern China［J］.Arch Virol，2013，158(8)：1783-1786.
［3］JACKWOOD M W.Review of infectious bronchitis virus around the world［J］.Avian Dis，2012，56(4)：634-641.
［4］胡北侠，杨少华，许传田，等.2008-2012年山东省鸡传染性支气管炎病毒遗传演化分析和致病性研究［J］.畜牧兽医学报，2014，45（5）：795-801.
HU B X， YANG S H， XU C T，et al.Phylogenetic analysis and pathogenicity of infectious bronchitis viruses isolated in Shandong province during 2008-2012［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(5)：795-801.(in Chinese)
［5］路希山，胡北侠，黄艳艳，等.2006-2008年山东鸡传染性支气管炎病毒分离株S1与N基因的分子特征［J］.畜牧兽医学报，2009，40(6)：879-885.
LU X S，HU B X，HUANG Y Y，et al.Molecular characterization of avian infectious bronchitis coronavirus strains isolated in Shandong between 2006 and 2008［J］.Acta Veterinaria et Zootechnica Sinica，2009，40(6)：879-885.(in Chinese)
［6］LI M，WANG X Y，WEI P，et al.Serotype and genotype diversity of infectious bronchitis viruses isolated during 1985-2008 in Guangxi，China［J］.Arch Virol，2012，157(3)：467-474.
［7］WANG C H，HUANG Y C.Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus［J］. Arch Virol，2000，145(2)：291-300.
［8］ARCHETTI I，HORSFALL F L Jr.Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum［J］.J Exp Med，1950，92(5)：441-462.
［9］杨汉春.动物免疫学［M］.北京：中国农业出版社，1999：146-147.
YANG H C.Animal Immunology ［M］.Beijing：China Agricultural University Press，1999:146-147.(in Chinese)
［10］MONDAL S P，LUCIO-MARTÍNEZ B，NAQI S A.Isolation and characterization of a novel antigenic subtype of infectious bronchitis virus serotype DE072［J］.Avian Dis，2001，45(4)：1054-1059.
［11］BROOKSBY J B.Variants and immunity：definitions for serological investigation［C］// International Symposium on Foot-and-Mouth disease：Variants and Immunity，Lyon，1967.Lyon，France.Symposia Series Immunobiological Standardization，1967，8：1-10.
［12］COWEN B S，HITCHNER S B.Serotyping of avian infectious bronchitis viruses by the virus-neutralization test［J］.Avian Dis，1975，19(3)：583-595.
［13］WINTERFIELD R W，FADLY A M.Some characteristics of isolates of infectious bronchitis virus from commercial vaccines［J］.Avian Dis，1972，16(4)：746-755.
［14］CAVANAGH D，DAVIS P J，COOK J K.et al.Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus［J］.Avian Pathol，1992，21(1)：33-43.
［15］IGNJATOVIC J，SAPATS S.Identification of previously unknown antigenic epitopes on the S and N proteins of avian infectious bronchitis virus［J］. Arch Virol，2005，150(9)：1813-1831.
［16］CHA C H，SOHN Y H，KO S Y，et al.Subgenotype and serotype analysis of hepatitis B virus in Korean chronic hepatitis B patients under treatment［J］.Korean J Lab Med，2009，29(1)：53-58.

[1]	YANG Zhenxing, MENG Jinxin, XIAO Lei, ZHU Jianbo, LIAO Defang, GAO Lin, LI Zhanhong, YANG Heng, LI Huachun. Isolation of Epizootic Haemorrhagic Disease Virus Serotype 7 Strain in China for the First Time [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 602-610.
[2]	LI Yanhong, LIU Jie, LI Weiping, CUI Yuting, PENG Yuanyi, WU Zhengli. Comparative Analysis of TLR4 Signaling Pathway in RAW264.7 Cells Activated by LPS from High-and Low-virulence Strains of Bovine Pasteurella multocida Serotype A [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 620-626.
[3]	LI Yanhong, LIU Jie, LI Weiping, CUI Yuting, PENG Yuanyi, WU Zhengli. Comparative Analysis of TLR4 Signaling Pathway in RAW264.7 Cells Activated by LPS from High-and Low-virulence Strains of Bovine Pasteurella multocida Serotype A [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 620-626.
[4]	YANG Zhenxing, MENG Jinxin, XIAO Lei, ZHU Jianbo, LIAO Defang, GAO Lin, LI Zhanhong, YANG Heng, LI Huachun. Isolation of Epizootic Haemorrhagic Disease Virus Serotype 7 Strain in China for the First Time [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 602-610.
[5]	LI Zhanhong, WANG Jinping, YANG Heng, LIAO Defang, SONG Jianling, GAO Lin, HE Yuwen, LI Huachun. Isolation of Bluetongue Virus Serotype 9 Strain in China for the First Time [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 354-363.
[6]	LI Zhanhong, WANG Jinping, YANG Heng, LIAO Defang, SONG Jianling, GAO Lin, HE Yuwen, LI Huachun. Isolation of Bluetongue Virus Serotype 9 Strain in China for the First Time [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 354-363.
[7]	CHEN Shenshen, MA Jiale, ZHANG Xinfeng, QU Xiangyang, PAN Zihao, YAO Huochun. Isolation and Identification of Streptococcus suis Causing Meningitis in Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 143-150.
[8]	CHEN Shenshen, MA Jiale, ZHANG Xinfeng, QU Xiangyang, PAN Zihao, YAO Huochun. Isolation and Identification of Streptococcus suis Causing Meningitis in Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 143-150.
[9]	GUO Jing-ying, LI Ya-fei, JIANG Hong-xia, ZENG Zhen-ling. Analysis and PFGE Genotyping of Serotype Ⅰ Riemerella anatipestifer in Guangdong Province [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1969-1978.
[10]	GUO Jing-ying, LI Ya-fei, JIANG Hong-xia, ZENG Zhen-ling. Analysis and PFGE Genotyping of Serotype Ⅰ Riemerella anatipestifer in Guangdong Province [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1969-1978.
[11]	YANG Heng, XIAO Lei, LI Zhan-hong, MENG Jin-xin, YANG Zhen-xing, Lü Min-na, LIN Xu-hui, LIAO De-fang, NIU Bao-sheng, LI Hua-chun. Sequence Analysis of Palyam Serogroup Virus Isolated in South China from 2012 to 2016 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 761-770.
[12]	YANG Heng, XIAO Lei, LI Zhan-hong, MENG Jin-xin, YANG Zhen-xing, Lü Min-na, LIN Xu-hui, LIAO De-fang, NIU Bao-sheng, LI Hua-chun. Sequence Analysis of Palyam Serogroup Virus Isolated in South China from 2012 to 2016 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 761-770.
[13]	WU Jing-bo, NAN Wen-jin, HUANG Jian-qiang, HU Hong-hui, PENG Guo-liang, PENG Ling, DONG Xiao-ying. Development of A Rapid Duplex Real-Time PCR Assay for Streptococcus suis and S. suis Serotype 2 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 368-377.
[14]	WU Jing-bo, NAN Wen-jin, HUANG Jian-qiang, HU Hong-hui, PENG Guo-liang, PENG Ling, DONG Xiao-ying. Development of A Rapid Duplex Real-Time PCR Assay for Streptococcus suis and S. suis Serotype 2 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 368-377.
[15]	Lü Min-na, YANG Heng, SUN Ming-fei, LI Juan, LIN Xu-hui, ZHANG Jian-fei, LIAO Shen-quan, QI Nan-shan, WU Cai-yan, LI Hua-chun, CHEN Qin-ling. Isolation and Identification of Bluetongue Virus Serotype 7 Field Isolate [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1281-1287.

Antigenic Relatedness and Serotype of Infectious Bronchitis Virus Isolated in Shandong Province

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments