蓝舌病病毒7型毒株的分离与分子鉴定

doi:10.11843/j.issn.0366-6964.2017.07.012

Abstract

Abstract:

In 2014, cattle blood samples were collected from Guangdong province and vector-borne pathogens contained in them were detected in the present study. Samples which were detected as BTV positive by quantitative reverse transcription polymerase chain reaction (qRT-PCR) were isolated and identified. Embryonated chicken eggs were subsequently inoculated and their livers were harvested to passage on C6/36 and BHK-21 cells, respectively. Extensive cytopathic changes were observed. A subsequent qRT-PCR assay target with VP 7 gene and SEM observation results further confirmed the presence of BTV, and named as GD/ST2014. In addition, VP 2 gene fragment was used for genotyping. And combined with VNT test results, the genotype of the isolate obtained in the present study were identified as BTV-7. This was the first report that bluetongue virus serotype 7 field isolate was isolated from cattle in China.

CLC Number:

S852.659.4

Lü Min-na, YANG Heng, SUN Ming-fei, LI Juan, LIN Xu-hui, ZHANG Jian-fei, LIAO Shen-quan, QI Nan-shan, WU Cai-yan, LI Hua-chun, CHEN Qin-ling. Isolation and Identification of Bluetongue Virus Serotype 7 Field Isolate[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1281-1287.

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Isolation and Identification of Bluetongue Virus Serotype 7 Field Isolate

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