新孢子虫GRA6表达、定位及对小鼠免疫效力的初步分析

doi:10.11843/j.issn.0366-6964.2015.04.016

Abstract

Abstract:

A variety of proteins are released during protozoan parasites invasion and growth in the host cell.Dense Granule protein is one of the key proteins which form and modify the parasitophorous vacuolar (PV).GRA6 gene was cloned from Neospora caninum genomic DNA.We constructed prokaryotic expression vector pGEX-GRA6 and eukaryotic expression vector pcDNA-GRA6.The protein was purified and immunized BALB/c mice to obtained high titer of serum.Results of indirect immunofluorescence (IFA) showed that GRA6 located at parasitophorous vacuolar network structure (PVN).BALB/c mice were immunized with recombinant protein or eukaryotic expression vector and challenged with Neospora caninum (2×10⁶).We monitored clinical symptoms and detected antibody levels of mice before each immunization.Thirty days after infection，brain burden of Neospora caninum was detected by RT-PCR.There were significant difference between immunization groups and control group (P<0.05)，which indicated that NcGRA6 had good immunoprotection for mice in both recombinant protein and eukaryotic expression vector.

Key words: Neospora caninum, GRA6, localization, immunogenicity

CLC Number:

S852.723

XING Ming，LIU Jing，WANG Hui，HAO Pan，LIU Qun. Neospora caninum GRA6 Recombinant Expression，Localization and Preliminary Analysis on the Mice Immune Effect[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 624-630.

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Neospora caninum GRA6 Recombinant Expression，Localization and Preliminary Analysis on the Mice Immune Effect

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