山羊肌内脂肪细胞lncRNA鉴别及特征分析

doi:10.11843/j.issn.0366-6964.2014.12.003

Abstract

Abstract:

The experiment was conducted to study the lncRNA functions in intramuscular adipocytes development in goats.We had identified lncRNA candidates combining CPAT with CPC，and analysed the sequence，structure and function through high-throughput RNA sequencing based on the transcriptome data from intramuscular preadipocytes and mature adipocytes.A total of 1 472 lncRNAs were obtained and 29 lncRNAs represented significant differential expression between intramuscular preadipocytes and mature adipocytes in goats.The data showed the lncRNAs had shorter transcript and ORF length and lower conservation than mRNA.Function analysis found that the nearby protein-coding genes were important to immune system，metabolism of lipids and lipoproteins，fatty acid，triacylglycerol and ketone body metabolism，etc.The differentially expressed lncRNAs are crucial to cell secretion，regulation of growth and cell morphology which have provided a new clue to research the mechanism of intramuscular adipocytes development.

Key words: lncRNA, goat, transcriptome, intramuscular adipocytes

CLC Number:

S827
S813.3

ZHENG Zhu-qing，DU Chen，FU Shao-yin，A NA，ZHANG Wen-guang. Identification and Characterization Analysis of Long Non-coding RNA from RNA-seq Data of Intramuscular Adipocytes in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 1924-1931.

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Identification and Characterization Analysis of Long Non-coding RNA from RNA-seq Data of Intramuscular Adipocytes in Goats

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