Kiss-1基因多态性与苏淮山羊产羔数的关联分析

doi:10.11843/j.issn.0366-6964.2014.12.002

Abstract

Abstract:

To investigate the effects of the polymorphisms of Kiss-1 gene on the reproductive traits of Suhuai goats，the mutations of Kiss-1 gene were screened using DNA pool technology，the polymorphism of Kiss-1 gene in 130 Suhuai goats were detected using AS-PCR and PCR-RFLP methods and its correlation with the litter size of Suhuai goats was analyzed.The results showed that 2 SNPs of g.2270C>T and g.2510A>G were found in Kiss-1 gene intron 1 sequence of Suhuai goats.Three genotypes(AA，AG and GG) were found at g.2510A>G locus in Suhuai goats，and the genotype frequency were 0.209，0.496 and 0.295，respectively；at g.2270C>T locus，3 genotypes(CC，CT and TT) were detected in Suhuai goats and the genotype frequency were 0.143，0.750 and 0.107，respectively.Association analysis showed that there was no significant difference among 3 genotypes (AA，AG and GG) of Suhuai goats in each parity litter size and average litter size at g.2510A>G locus；at g.2270C>T locus，the second parity litter size of CC genotype was significantly higher than those of CT (P<0.05) and CC genotypes(P<0.05).Haplotype analysis found 8 haplotypes and the first parity litter size of AGCT haplotype was significantly higher than that of AACT haplotype，and there existed no significant difference in litter size of Suhuai goats among other haplotypes.Therefore，it can be concluded that g.2270C>T locus of Kiss-1 gene may be a candidate molecular marker for reproductive traits selection in Suhuai goats.

Key words: Suhuai goats, Kiss-1 gene, PCR-RFLP, AS-PCR, haplotype, litter size

CLC Number:

S827
S813.3

LI Yin-xia，ZHANG Jun，QIAN Yong，LI Jing-xin，MENG Chun-hua,WANG Hui-li,ZHONG Sheng，CAO Shao-xian. Kiss-1 Gene Polymorphisms and Its Association with Litter Sizes in Suhuai Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 1917-1923.

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Kiss-1 Gene Polymorphisms and Its Association with Litter Sizes in Suhuai Goats

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