LEPR及LEP基因表达量对FecB不同基因型小尾寒羊产羔数的影响

doi:10.11843/j.issn.0366-6964.2018.05.010

Abstract

Abstract:

The aim of this study was to determine if the expression of leptin receptor (LEPR) and leptin (LEP) genes was associated with litter size of Small Tail Han ewes. Twenty one healthy Small Tail Han ewes, composed of 9 FecB wild type (++) and 12 FecB mutation homozygous type (BB), about 2-3 years old, were used to investigate the ovulation rate by laparoscopy after spontaneous estrus with CIDR. Real-time PCR was used to detect the expression of LEPR and LEP genes in 11 tissues (heart,liver,spleen,lung,kidney,adrenal gland, fallopian tube,uterus,hypothalamus,pituitary and ovary)of Small Tail Han ewes during follicular phase and in hypothalamus-pituitary-ovary gonadal axis of Small Tail Han ewes (++ and BB genotypes) during luteal phase and follicular phase. The results showed that both ovulation number (P=0.003 5) and litter size (P=0.004 0) of individuals with BB genotype were significantly higher than that of individuals with ++ genotype. These 2 genes were all expressed in 11 tissues during follicular phase, the expression of LEPR gene was high in ovary, uterus and adrenal gland, and the expression of LEP gene was high in hypothalamus, pituitary, ovary and kidney. In ovary, the expression of LEPR gene of individuals with ++ genotype during follicular phase was higher (P>0.05) than that during luteal phase. The LEPR expression of individuals with BB genotype during follicular phase was significantly higher than that of individuals with ++ genotype during follicular phase (P=0.007), BB genotype during luteal phase (P=0.003) and ++ genotype during luteal phase (P=0.003). Therefore, we concluded that there was a positive correlation between the high expression of LEPR gene during follicular phase and high litter size in Small Tail Han ewes, which provided a new clue for improving litter size in Small Tail Han sheep.

CLC Number:

S826.2

YU Jia-rui, WANG Xiang-yu, GUO Xiao-fei, HE Xiao-yun, SUN Qing, WEN Yu-liang, DI Ran, LIU Qiu-yue, HU Wen-ping, ZHANG Xiao-sheng, ZHANG Jin-long, SUN Wei, CHU Ming-xing. Effects of Expression of LEPR and LEP Genes on Litter Size of Small Tail Han Sheep with Different FecB Genotypes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 954-961.

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Effects of Expression of LEPR and LEP Genes on Litter Size of Small Tail Han Sheep with Different FecB Genotypes

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