北京油鸡肌肉和卵巢组织基因组DNA甲基化状态检测与分析

doi:10.11843/j.issn.0366-6964.2014.11.007

Abstract

Abstract:

The DNA methylation extent and pattern was assessed in genomes in muscle and ovary tissues from the 17-week-old Beijing You chicken using a methylation sensitive amplified polymorphism (MSAP) approach in this study.We analyzed different DNA methylation levels in different tissues.A total of 4 297 clear bands were obtained using 12 pairs of selective amplification primers，about 10-20 bands per sample with a pair of primers.The degree of methylation was approximate 35.86% in muscle and 31.50% in ovary in Beijing You chickens，and a significant difference was found between 2 tissues at the methylation levels (P<0.01).Further analysis indicated that no significant relationship was detected in ovary tissue between the methylation levels and individual weight (P>0.05)，but strongly negative correlation was depicted in muscle tissue (P<0.01).Five tissue-specific MSAP fragments were isolated and verified.Overall results reveal that there is a significant difference at the methylation levels between muscle and ovary tissues，and the methylated state of muscle tissue may play an important role in the body weight of Beijing You chicken.

Key words: methylation, tissues, weight, Beijing You chicken, MSAP

CLC Number:

S831.2
Q75

LI Jin-long，TANG Shao-qing，ZHAO Meng，WANG Hai-chao，XU Qing. Detection of DNA Methylation in Whole Genomes of Muscle and Ovary Tissues of Beijing You Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1784-1792.

References

［1］RAZIN A，CEDAR H.DNA methylation and gene expression ［J］.Microbiol Rev，1991，55(3)：451-458.
［2］JAENISCH R，BIRD A.Epigenetic regulation of gene expression：how the genome integrates intrinsic and environmental signals ［J］.Nat Genet，2003，33(Suppl)：245-254.
［3］JONES P A.Functions of DNA methylation：islands，start sites，gene bodies and beyond ［J］.Nat Rev Genet，2012，13(7)：484-492.
［4］BORGEL J，GUIBERT S，LI Y F，et al.Targets and dynamics of promoter DNA methylation during early mouse development ［J］.Nat Genet，2010，42(12)：1093-1100.
［5］GOKUL G，KHOSLA S.DNA methylation and cancer ［M］.Epigenetics：Development and Disease，2013：597-625.
［6］唐韶青，张沅，徐青，等.不同动物部分组织基因组甲基化程度的差异分析［J］.农业生物技术学报，2006，14(4)：507-511.
［7］MA J D，LI M Z，ZHOU S L，et al.Methylation-sensitive amplification polymorphism analysis of fat and muscle tissues in pigs ［J］.Genet Mol Res，2012，11(3)：3505-3510.
［8］XU Q，ZHANG Y，SUN D X，et al.Analysis on DNA methylation of various tissues in chicken ［J］.Anim Biotechnol, 2007，18(4)：231-241.
［9］田艳苓.鸡微卫星标记，DNA 甲基化差异和 FSHβ 变异及其与体重和产蛋性状的关系［D］.泰安：山东农业大学，2011.
［10］LU Y L，RONG T Z，CAO M J.Analysis of DNA methylation in different maize tissues ［J］.J Genet Genom，2008，35(1)：41-48.
［11］ZHANG M S，XU C M，WETTSTEIN D V，et al.Tissue-specific differences in cytosine methylation and their association with differential gene expression in sorghum ［J］.Plant Physiol，2011，156(4)：1955-1966.
［12］GEIMAN T M，MUEGGE K.DNA methylation in early development ［J］.Mol Reprod Dev，2010，77(2)：105-113.
［13］YANG C，ZHANG M J，NIU W P，et al.Analysis of DNA methylation in various swine tissues ［J］.PLoS ONE，2011，6(1)：e16229.
［14］GUPTA R，NAGARAJAN A，WAJAPEYEE N.Advances in genome-wide DNA methylation analysis ［J］.Biotechniques，2010，49(4)：iii-xi.
［15］XU M L，LI X Q，KORBAN S S.AFLP-based detection of DNA methylation ［J］.Plant Mol Biol Rep，2000，18(4)：361-368.
［16］GAO F，LUO Y L，LI S T，et al.Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls ［J］.PLoS ONE，2011，6(10)：e25901.
［17］DA K，NOWAK J，FLINN B.Potato cytosine methylation and gene expression changes induced by a beneficial bacterial endophyte，Burkholderia phytofirmans strain PsJN ［J］.Plant Physiol Biochem，2012，50(1)：24-34.
［18］LI X Q，XU M L，KORBAN S S.DNA methylation profiles differ between field-and in vitro-grown leaves of apple ［J］.J Plant physiol，2002，159(11)：1229-1234.
［19］DE MONTERA B，BOULANGER L，TAOURIT S，et al.Genetic identity of clones and methods to explore DNA ［J］.Cloning Stem Cells，2004，6(2)：133-139.
［20］YANG W，YU X，LIU B，et al.Parental epigenetic difference in DNA methylation-level may play contrasting roles for different agronomic traits related to yield heterosis in maize ［J］.Afr J Biotechnol，2011，10(46)：9253-9263.
［21］唐韶青.鸡生长与繁殖性状候选基因筛选及标记辅助选择的研究［D］.北京：中国农业大学，2010.
［22］徐青，张沅，孙东晓，等.应用MSAP方法检测鸡不同组织基因组的甲基化状态［J］.遗传，2011，33(6)：620-626.
［23］GRUNAU C，HINDERMANN W，ROSENTHAL A.Large-scale methylation analysis of human genomic DNA reveals tissue-specific differences between the methylation profiles of genes and pseudogenes ［J］.Hum Mol Genet，2000，9(18)：2651-2663.
［24］WAALWIJK C，FLAVELL R A.DNA methylation at a CCGG sequence in the large intron of the rabbit β-globin gene：tissue-specific variations ［J］.Nucleic Acids Res，1978，5(12)：4631-4642.
［25］XIONG L Z，XU C G，SAGHAI MAROOF M A，et al.Patterns of cytosine methylation in an elite rice hybrid and its parental lines，detected by a methylation-sensitive amplification polymorphism technique ［J］.Mol Gen Genet，1999，261(3)：439-446.
［26］PERAZA-ECHEVERRIA S，HERRERA-VALENCIA V A，ANDREW-JAMES K.Detection of DNA methylation changes in micropropagated banana plants using methylation-sensitive amplification polymorphism (MSAP) ［J］.Plant Sci，2001，161(2)：359-367.
［27］RIGGS A D.X inactivation，differentiation，and DNA methylation ［J］.Cytogenet Genome Res，1975，14(1)：9-25.
［28］刘健.DLY 猪不同部位脂肪和肌肉组织基因组甲基化程度的差异［D］.雅安：四川农业大学，2009.
［29］HASHIMSHONY T，ZHANG J M，KESHET I，et al.The role of DNA methylation in setting up chromatin structure during development ［J］.Nat Genet，2003，34(2)：187-192.
［30］ABASHT B，DEKKERS J C，LAMONT S J.Review of quantitative trait loci identified in the chicken ［J］.Poult Sci，2006，85(12)：2079-2096.
［31］UEMOTO Y，SATO S，ODAWARA S，et al.Genetic mapping of quantitative trait loci affecting growth and carcass traits in F2 intercross chickens ［J］.Poult Sci，2009，88(3)：477-482.
［32］PODISI B K，KNOTT S A，BURT D W，et al.Comparative analysis of quantitative trait loci for body weight，growth rate and growth curve parameters from 3 to 72 weeks of age in female chickens of a broiler-layer cross ［J］.BMC Genet，2013，14(1)：22.
［33］蒋曹德，邓昌彦，熊远著.猪个体 DNA 甲基化百分差异与胴体性状的关系［J］.农业生物技术学报，2005，13(2)：179-185.
［34］万亚琴.DNA 甲基化与肉牛杂种优势关系的初步研究［D］.重庆：西南大学，2008.
［35］SONG F，SMITH J F，KIMURA M T，et al.Association of tissue-specific differentially methylated regions (TDMs) with differential gene expression ［J］.Proc Natl Acad Sci USA，2005，102(9)：3336-3341.

[1]	ZHAO Yahan, HAO Haisheng, DU Weihua, PANG Yunwei, YAN Changliang, LIU Yan, ZHAO Shanjiang, ZHAO Xueming, ZHU Huabin. Study on Whole Genome Methylation Pattern of in vitro Fertilized Blastocysts from Fresh and Vitrified Bovine Oocytes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1179-1188.
[2]	ZHAO Yahan, HAO Haisheng, DU Weihua, PANG Yunwei, YAN Changliang, LIU Yan, ZHAO Shanjiang, ZHAO Xueming, ZHU Huabin. Study on Whole Genome Methylation Pattern of in vitro Fertilized Blastocysts from Fresh and Vitrified Bovine Oocytes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1179-1188.
[3]	ZHAN Siyuan, DING Xue, ZHONG Tao, WANG Linjie, LI Li, ZHANG Hongping. Comparison of IGF1R and IGF2R Expression Patterns in Different Tissues and Muscle Cells of Nanjiang Brown Goats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 701-711.
[4]	ZHAN Siyuan, DING Xue, ZHONG Tao, WANG Linjie, LI Li, ZHANG Hongping. Comparison of IGF1R and IGF2R Expression Patterns in Different Tissues and Muscle Cells of Nanjiang Brown Goats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 701-711.
[5]	LI Lanhui, DU Xiaolong, WANG Qi, GE Linhan, ZHANG Lechao, LI Xuemei, LI Xianglong. Exploration of Promoter Activity and Analysis of 5'UTR Sequence of Mink DCT Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 524-533.
[6]	LI Lanhui, DU Xiaolong, WANG Qi, GE Linhan, ZHANG Lechao, LI Xuemei, LI Xianglong. Exploration of Promoter Activity and Analysis of 5'UTR Sequence of Mink DCT Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 524-533.
[7]	WANG Yingming, XU Ya'ou, WANG Zhimin, XU Longyang, YANG Lei, LIN Yaqiu. Studies on the Cloning of KLF15 Gene, Tissue Expression Profile and the Association between Its Expression and Intramuscular Fat Content in Tibetan Chicken [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 261-270.
[8]	DU Xiaolong, WANG Qi, ZHANG Lechao, GE Linhan, LIU Xiaohui, WANG Han, LI Lanhui, LI Xianglong. Study on the Relationship between Methylation Level, SNP in Promoter Region of DCT Gene and Hair Color in Goat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 271-279.
[9]	WANG Yingming, XU Ya'ou, WANG Zhimin, XU Longyang, YANG Lei, LIN Yaqiu. Studies on the Cloning of KLF15 Gene, Tissue Expression Profile and the Association between Its Expression and Intramuscular Fat Content in Tibetan Chicken [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 261-270.
[10]	DU Xiaolong, WANG Qi, ZHANG Lechao, GE Linhan, LIU Xiaohui, WANG Han, LI Lanhui, LI Xianglong. Study on the Relationship between Methylation Level, SNP in Promoter Region of DCT Gene and Hair Color in Goat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 271-279.
[11]	WANG Shuang, YANG Hai-feng, YU Bo, LI Shi-yang, ZHENG Yu-ji, BU Shi-jin. Study on Residue Elimination of Altrenogest Oral Solution in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 2005-2014.
[12]	WANG Shuang, YANG Hai-feng, YU Bo, LI Shi-yang, ZHENG Yu-ji, BU Shi-jin. Study on Residue Elimination of Altrenogest Oral Solution in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 2005-2014.
[13]	DAI Chao-hui, FENG Hai-yue, ZONG Qiu-fang, WU Sheng-long, BAO Wen-bin. Relationship between Methylation in Promoter Region of TLR5 Gene and Resistance to Escherichia coli F18 of Sutai Weaned Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 897-906.
[14]	DAI Chao-hui, FENG Hai-yue, ZONG Qiu-fang, WU Sheng-long, BAO Wen-bin. Relationship between Methylation in Promoter Region of TLR5 Gene and Resistance to Escherichia coli F18 of Sutai Weaned Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 897-906.
[15]	CHANG Tian-peng, XIA Jiang-wei, BAO Jin-shan, JIN Sheng-yun, ZHU Bo, XU Ling-yang, CHEN Yan, ZHANG Lu-pei, GAO Xue, LI Jun-ya, GAO Hui-jiang. Genome-wide Association Study for Carcass Traits Using Two Statistic Models in Chinese Simmental Beef Cattle [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 833-840.

Detection of DNA Methylation in Whole Genomes of Muscle and Ovary Tissues of Beijing You Chicken

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments