不同毒力猪肺炎支原体侵入宿主细胞的观察

doi:10.11843/j.issn.0366-6964.2014.06.017

Abstract

Abstract:

This experiment was conducted to study the invasion and location in host cells of different virulent Mycoplasma hyopneumoniae.The ability of wild strain XLW-1 to invade PK15 cells at different infection time interval was investigated firstly by using indirect immunofluorescence technique and laser scanning confocal microscope.Then，the invasion to swine tracheal epithelial cells (STEC)，PK15，SJPL and 3D4/21 of different virulent M.hyopneumoniae strains was observed.The results showed that 1) M.hyopneumoniae wild strains XLW-1 can intrude into PK15 cells up to 10 h post infection and no internalization was observed before 8 h; 2) The amount of intracellular M.hyopneumoniae XLW-1 was increased as infection time; 3) M.hyopneumoniae wild strainsXLW-1，168 attenuated strains and vaccine strains all could invade STEC，PK15，SJPL and 3D4/21 cells; 4) The ingested wild strainsXLW-1，168 attenuated strains and vaccine strains were randomly distributed in the cytoplasm of STEC，and mainly distributed in the cytoplasm of long and narrow two polars and cytoplasm near cytomembrane in PK15; The internalized wild strainsXLW-1 and 168 vaccine strains principally concentrated in the cytoplasm close to medial surface of SJPL cells membrane，but 168 attenuated strains randomly interspersed in the cytoplasm of SJPL cells; The incursive wild strainsXLW-1 and 168 attenuated strains principally distributed in the cytoplasm close to inboard surface of 3D4/21 cytomembrane，and 168 vaccine strains randomly spreaded in the cytoplasm of 3D4/21 cells.These results demonstated that M.hyopneumoniae of different virulence all can invade host cells STEC，PK15，SJPL and 3D4/21.

Key words: Mycoplasma hyopneumoniae, different virulence, host cells, invasion

CLC Number:

S852.62

CHE Qiao-lin，LIU Bei-bei，LIU Mao-jun，XIONG Qi-yan，SHAO Guo-qing. Invasion of Host Cells by Different Virulent Mycoplasma hyopneumoniae[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 981-988.

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Invasion of Host Cells by Different Virulent Mycoplasma hyopneumoniae

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