组氨酸标签标记的口蹄疫重组病毒的制备及鉴定

doi:10.11843/j.issn.0366-6964.2014.06.014

Abstract

Abstract:

To identify a feasible insertion site in the foot-and-mouth disease virus (FMDV) and obtain a tagged recombinant virus，in this study，we have used reverse genetics to introduce a histidine (His)tag into the ninth site in the downstream of RGD motif in FMDV and obtain His tagged full-length-cDNA clone， named prAsia1-9His.We transfected the prAsia1-9His into BHK-21 cells and typical cytopathic effect (CPE) were found in the transfacted BHK-21 cells.Then，RT-PCR，indirect immunofluorescence assay and sequence analysis were performed，the results showed that the recombinant virus named as rAsia1-9His was successfully rescued and the His tag was stably maintained.Finally，we compared rAsia1-9His with the parental virus (named rAsia1) for pathogenicity to BHK-21 cells and suckling mouse，the results showed that they had similarity in the biological characteristics.The successful rescue of tagged FMDV lays a foundation for further study of molecular pathogenesis and marker vaccine of FMDV.

Key words: foot and mouth disease (FMD), tag, marker vaccine

CLC Number:

S852.659.

YANG Bo，YANG Fan，WANG Song-hao，ZHANG Yan，YUE Cheng,ZHENG Hai-xue，YIN Hong. Generation and Identification of a Histidine Tagged Recombinant Foot and Mouth Disease Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 960-966.

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Generation and Identification of a Histidine Tagged Recombinant Foot and Mouth Disease Virus

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