不同猪源脑心肌炎病毒的分离、鉴定和全基因组序列分析

doi:10.11843/j.issn.0366-6964.2014.06.015

Abstract

Abstract:

The current study was undertaken to study the infection status of EMCV from different pig breed.Modified “cell inoculation and RT-PCR” technology was used，and the 5 EMCV strains were isolated from local pig，domestic boar and improved breed pig.The full length genome of the EMCV strains were acquired and sequenced，and their molecular characteristic were analyzed.The results showed that the full-genome sequence of 5 EMCV isolates were 7724-7735 bp in length，and shared 99.3%-99.8% nucleotide identity each other，79.9%-99.9% identity with reference EMCV strains from different animal sources，and 99.4% with Chinese strains from pig and mice.Among all gene fragments，VP1 and 2A are easy to mutation，VP2 and 3D are most conserved.The phylogenetic tree based on the full length genome，ORF and VP1 gene sequences showed that the EMCV were divided into G1，G2 and G3 groups.The isolates from pig belong to G1 and G2 groups，the ones from mice distribute in G1 and G3 group，5 isolates strains in this study belong to G1 group with other Chinese reference strains.The results identified that the EMCV infection could cause severe clinical symptoms in local pig and domestic boar，which remind us of taking fully consideration of the zoonosis ecology in the course of Wild animal breeding activities.Mice may play an important medium role in incross infection of EMCV at the different pig breed.A big regional difference exists in EMCV and the transmission is limited in a range of area，moreover，some mutation may occur in EMCV infection to adapt new hosts.

Key words: encephalomyocarditis virus, different pig breed, RT-PCR, full length genome, molecular characterization analysis

CLC Number:

S852.659.6

CHANG Hong-tao，LIU Hui-min，CHEN Lu，YANG Xia，ZHAO Jun，WANG Xin-wei，YAO Hui-xia，WANG Chuan-qing. Isolation，Identification and Full Length Genome Sequence Analysis of EMCV from Different Pig Source[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 967-973.

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Isolation，Identification and Full Length Genome Sequence Analysis of EMCV from Different Pig Source

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