EYA 3和TSH β在季节性发情和常年发情绵羊中的表达模式

doi:10.11843/j.issn.0366-6964.2018.02.005

Abstract

Abstract:

The aim of this study was to reveal EYA 3 and TSH β regulatory roles in seasonal estrus and reproduction stages transition.we investigated the expression patterns of eyes absent 3 (EYA 3) and thyrotropin beta subunit (TSH β) genes in different tissues of seasonal estrous sheep(Sunite sheep, SNT)at different photoperiods (long photoperiod (LP) and short photoperiod(SP)) and year-round estrous sheep (Small Tail Han sheep, STH) at different reproduction stages (luteal phase and follicular phase) through q-PCR technology. The results showed that EYA 3 was widely expressed in different tissues of 2 breeds, while TSH β was expressed highly in pituitary. In most tissues, the expression levels of those 2 genes under long photoperiod (LP) were higher than those under short photoperiod (SP) in SNT, and the expression levels were higher at luteal phase than those at follicular phase in STH. When SP turned into LP in SNT, the expression levels of those 2 genes rose in pituitary, and EYA 3 showed a transient peak at LP 3, and the peak of TSH β was at LP 21. The expression of EYA 3 declined prior to TSH β. The results elucidated the expression characteristics of EYA 3 and TSH β in year-round estrous and seasonal estrous sheep, the expression characteristics of those 2 genes at different photoperiods and different reproduction stages in pineal and pituitary of sheep, which suggested that EYA 3 played an important role in pineal and might regulate seasonal estrous of sheep. In addition, EYA 3 and TSH β might be involved in the transition of estrous stages. When SP turned to LP, EYA 3 played a regulatory role earlier than TSH β.

CLC Number:

S826.2

XIA Qing, ZHANG Xiao-sheng, LIU Qiu-yue, WANG Xiang-yu, HE Xiao-yun, GUO Xiao-fei, HU Wen-ping, ZHANG Jin-long, CHU Ming-xing, DI Ran. Expression Patterns of EYA 3 and TSH β in Seasonal Estrous and Year-round Estrous Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 263-269.

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Expression Patterns of EYA 3 and TSH β in Seasonal Estrous and Year-round Estrous Sheep

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