东北地区野生鸟类J亚群禽白血病分子流行病学调查及部分基因组序列分析

Abstract

Abstract:

In order to analyze the situation of wild birds infected avian leukosis virus(ALV) in China in 2010-2011, we collected 300 samples of wild birds from different areas. Seven strains of ALV-J were detected by PCR. The env gene and the 3′UTR were amplified, cloned and sequenced from seven isolated strains, which were subgroup J-positive. The results showed that the env genes of the seven strains from positive samples were not in the same branch with prototype strain HPRS-103 and representative strains, and the 3′UTR of them were a little different from HPRS-103. Part sites of E element have mutation, compared with HPRS-103. ALV has infected the wild birds in China. The large circumscription of the wild birds increases the difficulty of prevention and control of AL, though the relevance ratio is low. Parts of the env gene and 3′UTR of isolated strains have varied by sequencing and analyzing. More attention should be paid in preventing and controlling the transmission of ALV among wild birds.

Key words: wild birds, avian leukosis, epidemiological survey, sequence analysis

CLC Number:

S852.659.3

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LI De-long, GAO Yu-long, ZENG Xiang-wei, YANG Bo, LIU Wan-si, GAO Qi, QIN Li-ting, GAO Hong-lei, WANG Xiao-mei, LIU Si-dang. Molecular Epidemiology and Part Genome Sequence Analysis of Subgroup J Avian Leukosis Virus Isolated from Wild Birds in Northeast China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA.

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Molecular Epidemiology and Part Genome Sequence Analysis of Subgroup J Avian Leukosis Virus Isolated from Wild Birds in Northeast China

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