ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (12): 1735-1740.doi:

• 遗传繁育 • Previous Articles     Next Articles

Comparison of Somatic Cell Nuclear Transfer of Buffalo and the Effects of Time Interval before Activation on Nuclear Transfer by WholeCell Intracytoplasmic Microinjection

YANG Sufang 1, CHEN Zihong 1,2, WEI Jingwei 1, LU Fenghua 1, SHI Deshun 1*   

  1. 1.Animal Reproduction Institute, Guangxi University, Nanning 530005, China; 2.Population and Family Planning Research Centre of Guangxi Zhuang Autonomous Region, Nanning 530021, China
  • Received:2008-12-25 Revised:1900-01-01 Online:2009-12-24 Published:2009-12-24
  • Contact: SHI Deshun

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Abstract: The aim of the study was to compare two methods of somatic cell nuclear transfer(SCNT), and explore the effects of time interval before activation on nuclear transfer(NT) by WholeCell Intracytoplasmic Microinjection (WCICSI). NT effect of Perivitelline Microinjection (PM) and WCICSI are compared, when the fetus fibroblasts were used for donor cells. And effect of activation time interval after donor cell by WCICSI on buffalo SCNT was also examined. The results showed that the embryo reconstructing efficiency by WCICSI was higher than that by PM (87.1% vs 81.1%, P<0.05), but the cleavage rate of reconstructed embryos was lower in former than that in latter (49.5% vs 71.8%, P<0.01). The blastocyst development rate of reconstructed embryos and the total efficiency of nuclear transfer were not significantly different each other (21.6% vs 19.3%, P>0.05;18.8% vs 15.7%, P>0.05). The blastocyst development rate of reconstructed embryos derived from cumulus cells and fibroblasts were the highest when the reconstructed embryos were activated at 3 h after injection. There was no significant difference in the development of blastocyst derived from granular cells between the time interval from 1.5 to 3 h, but the best time interval before activation was 3 h. The results indicated the WCICSI method can be employed in buffalo SCNT. Development rate of reconstructed embryos is best when activation at 3 h after buffalo cytoplasmic injection of donor cells.