Abstract: To identify the fragment cDNA encoding alpaca Bclaf1, and study the expression characteristic of the Bclaf1 in alpaca skin, a cDNA library was screened from alpaca skin and ESTs were analyzed, the cellular localization of Bclaf1 in alpaca skin was studied by immunohistochemistry. The multiple sequence alignments revealed that the similarity of Bclaf1 of alpacas was 100%, 92.9%, 86.0%, 81.1% and 77.9% to Bclaf1 of rattus norvegicu, mus, canis families, homo sapenis, pan troglodytes, respectively. The result showed that no positive cells were found in hair follicles of juvenile alpaca, the positive expression was focused in outer rootsheath of hair follicles of adult alpaca, white alpaca and brown alpaca; the expression in hair follicles was significantly different between the white and brown alpacas based on the average optical density. The expression and localization of Bclaf1 in alpaca skin were changed with age and coat color pattern.