Abstract: In this study, the molecular properties and potential functions of porcine TIMP2 gene were analyzed and characterized in Wuzhishan laboratory miniature pig. Porcine TIMP2 cDNA was obtained by reverse transcription polymerase chain reaction (RTPCR), using in silico cloning strategy based on porcine ESTs database, the nucleotide and amino acid sequences were characterized with bioinformatics methods, and its expression patterns in different tissues and development stages were also studied by RTPCR. The length of the cloned porcine TIMP2 cDNA was 909 bp including a 663 bp entire open reading frame (ORF), which encodes a 221 amino acids protein. Multiple sequences alignment and phylogeny analysis indicated that porcine TIMP2 protein shared high similarity with that of other species, such as Homo sapiens (97%), Mus musculus (97%) and Rattus norvegicus(97%). Protein sequence analysis showed that the deduced porcine TIMP2 protein sequence (pI=7.65， MW= 24.5 ku) contained 27AA leader sequence and 194 AA mature peptide, the leader sequence in pig has one more amino acid (Leu residue) than that in other 6 species. A NTRTIMP function domain and a conserved VIRAK motif at Nterminus were predicted by bioinformatic analysis and homologous comparison, and the 12 Cys residues in the sequence were predicted to constitute 6 disulphide bonds. The expression of porcine TIMP2 mRNA varied greatly in different tissues as well as different developmental stages, it was highly expressed in testis, pituitary body, stomach, large intestine, ovary, uterus and 90 days embryonic longissimus muscle, but lowly in heart, small intestine, brain, liver and adult longissimus muscle, and a moderate expression level was detected in kidney, thymus, spleen, thyroid and 33 days embryonic longissimus muscle. The result indicated that the porcine TIMP2 mRNA was highly expressed in organs and phases with relatively high tissue growth and/or remodelling activities, and it was in accordance with the innate biological function of TIMP-2.