ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (4): 391-396.doi:

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The Construction and Screening of cDNA Expression Library of Oncosphere of Taenia solium and cloning of Immunogen Gene

JING Zhi-zhong;GUO Ai-jiang;HAI Gang;ZONG Rui-qian;CAI Xue-peng   

  1. Key Laboratory of Agricultural Ministry, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences， Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-04-25 Published:2005-04-25

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Abstract: The cDNA expression library of the Oncosphere development stage was constructed in order to avoid involved in large genome of Teania solium. The results showed that the transformation efficacy and recombinant rate of library were 5.0×106 and 100%, respectively. The genes of 10 ku, 18 ku and A, B, C types of 45W were cloned from the cDNA library, which approved the library possessed a good quality and high representation. The library was screened with antiserum, and gene of TsO1 was cloned and sequenced. ORF size of TsO1 was 393 bp. The sequance of TsO1 was submited to GenBank， and the number AY327451 was given. It has been proved the TsO1 is a novel gene of the Oncosphere development stage by analysis of BLAST.