ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (4): 387-390.doi:

• 兽医 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the H11 Antigen Gene of Haemonchus contortus ZJ strain

DU Ai-fang;LI Xiao-jun;HOU Yu-hui;WANG Su-hua   

  1. Institute of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-04-25 Published:2005-04-25

Abstract: In order to clone the H11 gene, the total RNA of Haemonchus contortus ZJ strain was extracted and used as template of RT-PCR. A pair of primers was designed according to the published data. After RT-PCR, the cloned H11 was ligated to the vector pUCm-T and transformated DH5α competent cell. The positive clones were verified by sequencing. Sequence analysis showed that the gene obtained was 99.5% identical to the H11 gene in the database. According to the sequence analysis, the deduced protein of H11 may has the function similar to aminopeptidase which make parasites dead from lack of necessary amino acids.