罗伊氏乳杆菌对仔猪空肠miRNAs表达谱的影响

doi:10.11843/j.issn.0366-6964.2020.09.013

Abstract

Abstract: This study was conducted to investigate the effect of Lactobacillus reuteri on the expression of miRNAs in the jejunum of piglets. Six litters of healthy 1-day-old York×Rongchang piglets were selected and randomly divided into the two group，3 litters per group(n=27). The two groups were control group (daily orally adminitrated with 0.1% sterile peptone solution per piglet)，and the LR group (daily orally administrated with 1.0×10¹⁰ CFU of L. reuteri per piglet)， respectively. At 10 and 20 days of age, six piglets per group were selected for slaughter, respectively, and the jejunum samples were collected. After RNA extraction, the library was constructed and the miRNA expression profile of the jejunum was detected by Solexa high-throughput sequencing technology. Bioinformatics technology was used to conduct target genes prediction and functional analysis. The results showed that: 1) A total of 8 libraries were constructed and a total of 187 103 840 clean reads were obtained, of which the 22 nt length sequence accounted for the largest proportion; 2) Three hundred and fifty-four and 352 known miRNAs were respectively identified at 10 and 20 days of age, of which 329 miRNAs were co-expressed; 3) Differentially expressed (DE) miRNAs in the jejunum were found after L. reuteri administration, 7 and 9 DE miRNAs were obtained at 10 and 20 days of age，respectively. Notably, ssc-miR-218 was expressed differentially at two ages. 4) KEGG pathway analysis and target gene prediction on the differentially expressed miRNAs revealed that 10 221 target genes were enriched in 293 biological pathways, of which 66 were significantly enriched (P<0.05), including the MAPK signaling pathway and PI3K-Akt signaling pathway, which were known to regulate intestinal development and immune. 5) Six miRNAs were selected to validate the sequencing results by qRT-PCR, the qRT-PCR expression results corresponded well with those from the sequencing. The results indicates that L. reuteri may regulate intestinal health related pathways by influencing the expression of jejunal miRNAs.

Key words: Lactobacillus reuteri, miRNA, Solexa high-throughput sequencing, jejunum, piglet

CLC Number:

S828

SUN Qian, WANG Qi, HUANG Jinxiu, QI Renli, YANG Feiyun, WANG Ruisheng, LAN Yunxian. Effect of Lactobacillus reuteri on the Expression Profile of miRNAs in the Jejunum of Piglets[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2156-2164.

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Effect of Lactobacillus reuteri on the Expression Profile of miRNAs in the Jejunum of Piglets

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