维生素D介导鸡BMSCs和BMMs共培养体系中破骨细胞的形成

doi:10.11843/j.issn.0366-6964.2019.07.021

Abstract

Abstract: This study aimed to investigate the effect of 1α, 25-(OH)₂D₃ on the formation of osteoclast (OC) in the co-culture system of chicken bone marrow mesenchymal stem cells (BMSCs) and bone marrow mononuclear macrophage cells (BMMs). In the study, tartrate-resistant acid phosphatase (TRAP), bone absorption function identification, and OC marker mRNA and protein expression detection were performed on cells treated with 1α, 25-(OH)₂D₃. The results showed that, 1α, 25-(OH)₂D₃ up-regulated the expression of receptor activator for nuclear factor-κB ligand (RANKL) and down-regulated the expression of osteoprotegerin (OPG) of BMSCs. Compared to the control group, the number of OC was increased in the co-culture system at day 5 treated by 1α, 25-(OH)₂D₃. Meanwhile, the bone resorption activity, the mRNA and protein expression of matrix metalloproteinase 9 (MMP-9), carbonic anhydrase Ⅱ (CAⅡ), cathepsin K and TRAP were significantly enhanced (P<0.01) by 1α, 25-(OH)₂D₃. Among them, 10^-8 mol·L^-11α, 25-(OH)₂D₃ had the most obvious effect. The results confirmed that 10^-8 mol·L^-1 1α, 25-(OH)₂D₃ could mediate the formation of OC, which showed bone resorption activity, in the co-culture system of BMSCs and BMMs from chicken. Our study lays a foundation for further research on the mechanism of poultry OC in bone, calcium and phosphorus metabolic diseases.

CLC Number:

S856.5

GU Jianhong, MIN Wenyan, ZHOU Jiahua, ZHAO Yutian, BIAN Jianchun, LIU Xuezhong, YUAN Yan, LIU Zongping. Vitamin D Regulates OC Formation in the Co-culture System of Chicken BMSCs and BMMs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1509-1517.

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Vitamin D Regulates OC Formation in the Co-culture System of Chicken BMSCs and BMMs

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