术苦芩总多糖对湿热泄泻仔猪小肠杯状细胞数量以及MUC-2和ITF-3 mRNA转录的影响

doi:10.11843/j.issn.0366-6964.2019.06.021

Abstract

Abstract: To investigate the effect of Polysaccharides from ZhuKuQin (ZKQPs) on the recovery function in damp-heat diarrhea Piglet intestine, 60 piglets were divided into blank control group (n=10) and damp-heat diarrhea model group (high temperature, humidity environment combined with high fat compound feed), and 50 model piglets were randomly divided into model group, positive drug group (Pulsatilla powder), ZKQPs high, middle and low dose group, with 10 piglets in each group, drugs were mixed with feed, for 7 days. Small intestinal goblet cells (GC) were stained with PAS and observed by Microscope, RT-PCR and ELISA were used to detect the transcriptional quantity and content of mucoprotein-2 (MUC-2) and intestinal trilobular factor (ITF-3) in intestinal mucus of damp-heat diarrhea piglets. The results were as follows:1)The number of GC in duodenum, jejunum and ileum of piglets in model group decreased significantly when compared with that in blank group (P<0.05); The number of GC in duodenum, jejunum and ileum of piglets in ZKQPs high, middle dose group and positive drug group increased. The number of GC in duodenum, jejunum and ileum of piglets in the ZKQPs high dose group and the positive drug group were significantly different when compared with the model group (P<0.05), and the number of GC in duodenum and ileum of piglets in ZKQPs middle dose group was significantly different (P<0.05). 2)The transcriptional quantity and content of MUC-2 in duodenum, jejunum and ileum of piglets were decreased in model group, and the difference was significant when compared with that of blank group (P<0.05).The transcriptional quantity and content of MUC-2 in duodenum, jejunum and ileum of piglets in each ZKQPs dose groups and positive drug groups increased. The transcriptional quantity and content of MUC-2 in duodenum, jejunum and ileum of piglets in ZKQPs high dose group and positive drug group were significantly different from those in model group (P<0.01). 3)The transcriptional quantity and content of ITF-3 in duodenum, jejunum and ileum of piglets were decreased in model group, and the difference was significant when compared with that of blank group (P<0.05). The transcriptional quantity and content of ITF-3 in duodenum, jejunum and ileum of piglets in ZKQPs high, middle dose group and positive drug group increased.The transcriptional quantity and content of ITF-3 in jejunum and ileum of piglets in ZKQPs high, middle dose group and positive drug group were significantly different from those in model group (P<0.01). ZKQPs can increase the number of GC and the transcription of MUC-2 and ITF-3 mRNA in small intestine of damp-heat diarrhea Piglets, and promote the secretion of MUC-2 and ITF-3 in small intestine of damp-heat diarrhea Piglets.

CLC Number:

S853.74

LIN Chunfa, HAO Yongfeng, LIU Juan. Effect of Polysaccharides from ZhuKuQin on the Number of Intestinal Goblet Cell and the Transcription of MUC-2 and ITF-3 mRNA in the Small Intestine of Damp-heat Diarrhea Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1301-1311.

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Effect of Polysaccharides from ZhuKuQin on the Number of Intestinal Goblet Cell and the Transcription of MUC-2 and ITF-3 mRNA in the Small Intestine of Damp-heat Diarrhea Piglets

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