林麝源屎肠球菌LS170308株多重耐药基因岛PRI1分析

doi:10.11843/j.issn.0366-6964.2019.06.018

Abstract

Abstract: Enterococcus faecium has been reported as a conditional pathogen in clinical practice, mainly causing endocarditis and sepsis in the host. At present, the research on drug resistance of E. faecium has become increasingly prominent, mainly because the bacteria are natural resistant to β-lactam and its resistant ability to glycopeptide antibiotics (such as vancomycin) are increasing, but the drug resistance transmission mechanism of this strain is still under study. The purpose of this study was to describe the characteristics of a multidrug resistance genomic island from E. faecium. Highlight the spread of the genomic island in different genera of bacteria. At the same time, it provides data that support for the spread of bacterial resistance in wild animals. Single-molecule sequencing technology (de nove) was used in this E. faecium, and the genomic island was analyzed using all kinds of molecular biology tools. Results were as follows:This strain of E. faecium carries multiple resistance genes on its chromosome; The Tn554 transposase carrying ANT(9) and ErmA and the pair of intercalating elements IS256 carrying AAC(6')-le-APH(2″)-la together constitute a multidrug resistance genomic island from Staphylococcus aureus. The genomic island is resistant to macrolides, lincosamides and aminoglycoside antibiotics. In conclusion, a strain of E. faecium was isolated from the visceral tissue of dead forest carp, which carries a multi-drug resistant genomic island PRI1. This genomic island is currently not reported in E. faecium. At the same time, this strain is derived from wild animals, so the resistance of wild animals has become more and more serious.

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GONG Yongping, CHEN Zhenrong, YIN Wenqi, WEN Jifeng, YI Keke, YAN Qigui. Analysis of Multidrug-resistant Genomic Island PRI1 of Enterococcus faecium LS170308 from Moschus berezovskii[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1275-1283.

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Analysis of Multidrug-resistant Genomic Island PRI1 of Enterococcus faecium LS170308 from Moschus berezovskii

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