猪胎盘特异基因1(PLAC1)的克隆、组织表达及遗传方式研究

doi:10.11843/j.issn.0366-6964.2019.01.005

Abstract

Abstract:

The objective of this study was to investigate the expression of porcine PLAC1 gene,and preliminarily identify its gene structure, biological function and inheritance patterns. The placenta tissue of pigs on 26, 50 and 95 days of pregnancy were collected, the full-length of PLAC1 mRNA and its splicing variants were cloned by using the RACE-PCR(rapid-amplification of cDNA ends) and RT-qPCR(real-time quantitative PCR). Moreover, the expression patterns of PLAC1 gene in placenta of pigs was verified by in situ hybridization. In addition, the sequence variation of PLAC1 gene was detected. The results showed that there were 3 different transcripts of the PLAC1 gene in pigs, and the CDS regions of the 3 transcripts were identical, with a length of 525 bp, encoding 174 amino acids; The results of the expression profile and hybridization in situ of PLAC1 gene indicated that the PLAC1 gene was specifically expressed in pig placental chorionic epithelium, and the expression of PLAC1 mRNA was different during different stages of pregnancy. The expression of PLAC1 mRNA was significantly higher on 50 and 95 days of gestation in placenta of pigs than 26 days of gestation. The genotype of SNP on PLAC1 gene confirmed that it was a hemizygous gene, and followed the inheritance patterns of hemizygous gene. The experiment data provide a basis for exploring the biological function of porcine PLAC1 gene in placenta development.

CLC Number:

S828.2

DENG Dadong, HONG Linjun, PAN Li, LIU Bang, YU Mei. Cloning, Tissue Expression and Inheritance Patterns of Porcine Placenta-specific 1(PLAC1) Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 37-43.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2019.01.005

https://www.xmsyxb.com/EN/Y2019/V50/I1/37

References

[1] ZHOU Y F,XU T,CAI A L,et al.Excessive backfat of sows at 109 d of gestation induces lipotoxic placental environment and is associated with declining reproductive performance[J].J Anim Sci,2018,96(1):250-257.
[2] CHEN F,WANG T J,FENG C P,et al.Proteome differences in placenta and endometrium between normal and intrauterine growth restricted pig fetuses[J].PLoS One,2015,10(11):e0142396.
[3] RAWN S M,CROSS J C.The evolution,regulation,and function of placenta-specific genes[J].Annu Rev Cell Dev Biol,2008,24(1):159-181.
[4] COCCHIA M,HUBER R,PANTANO S,et al.PLAC1,an Xq26 gene with placenta-specific expression[J].Genomics,2000,68(3):305-312.
[5] FANT M,BARERRA-SALDANA H,DUBINSKY W,et al.The PLAC1 protein localizes to membranous compartments in the apical region of the syncytiotrophoblast[J].Mol Reprod Dev,2007,74(7):922-929.
[6] LIM J H,KIM S P,GABRIELSON E,et al.Activation of human cancer/testis antigen gene,XAGE-1,in tumor cells is correlated with CpG island hypomethylation[J].Int J Cancer,2005,116(2):200-206.
[7] SUEMIZU H,AIBA K,YOSHIKAWA T,et al.Expression profiling of placentomegaly associated with nuclear transplantation of mouse ES cells[J].Dev Biol,2003,253(1):36-53.
[8] HEMBERGER M C,PEARSALL R S,ZECHNER U,et al.Genetic dissection of X-linked interspecific hybrid placental dysplasia in congenic mouse strains[J].Genetics,1999,153(1):383-390.
[9] FANT M E,FUENTES J,KONG X Y,et al.The nexus of prematurity,birth defects,and intrauterine growth restriction:a role for Plac1-regulated pathways[J].Front Pediatr,2014,2:8.
[10] CHANG W L,WANG H Y,CUI L,et al.PLAC1 is involved in human trophoblast syncytialization[J].Reprod Biol,2016,16(3):218-224.
[11] GU Y,WAN J,YAO L,et al.Plac1 expression pattern at the mouse fetomaternal interface and involvement in trophoblast differentiation[J].Cell Physiol Biochem,2017,43(5):2001-2009.
[12] JACKMAN S M,KONG X Y,FANT M E.Plac1(placenta-specific 1) is essential for normal placental and embryonic development[J].Mol Reprod Dev,2012,79(8):564-572.
[13] ROSS J W,ASHWORTH M D,STEIN D R,et al.Identification of differential gene expression during porcine conceptus rapid trophoblastic elongation and attachment to uterine luminal epithelium[J].Physiol Genomics,2009,36(3):140-148.
[14] BISCHOFF S R,TSAI S Q,HARDISON N E,et al.Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins[J].PLoS One,2013,8(1):e55345.
[15] HONG L J,HOU C Y,LI X P,et al.Expression of heparanase is associated with breed-specific morphological characters of placental folded bilayer between Yorkshire and Meishan pigs[J].Biol Reprod,2014,90(3):Article 56.
[16] LIU R Z,WANG M,SU L J,et al.The Expression pattern of microRNAs and the associated pathways involved in the development of porcine placental folds that contribute to the expansion of the exchange surface area[J].Biol Reprod,2015,93(3):Article 62.
[17] BAZER F W,THATCHER W W,MARTINAT-BOTTE F,et al.Sexual maturation and morphological development of the reproductive tract in large white and prolific Chinese Meishan pigs[J].J Reprod Fertil,1988,83(2):723-728.
[18] YUAN H Y,WANG X Y,SHI C M,et al.Plac1 is a key regulator of the inflammatory response and immune tolerance in mammary tumorigenesis[J].Sci Rep,2018,8(1):5717.
[19] LI Y F,CHU J H,LI J,et al.Cancer/testis antigen-Plac1 promotes invasion and metastasis of breast cancer through Furin/NICD/PTEN signaling pathway[J].Mol Oncol,2018,12(8):1233-1248.
[20] YANG L,ZHA T Q,HE X,et al.Placenta-specific protein 1 promotes cell proliferation and invasion in non-small cell lung cancer[J].Oncol Rep,2018,39(1):53-60.

Cloning, Tissue Expression and Inheritance Patterns of Porcine Placenta-specific 1(PLAC1) Gene

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	NIU Naiqi, ZHAO Runze, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 79-86.
[2]	ZHU Xueli, ZHANG Longchao, WANG Lixian, PU Lei, LIU Xin. Association Analysis of AQP9 and RPS10 Gene Polymorphisms with Backfat Thickness in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 87-98.
[3]	YANG Kai, LU Zhuoda, HE Jian, ZHANG Ruiqi, WANG Suqing, LI Kebiao, ZHAO Yunxiang, ZHU Xiaoping, GUO Jinbiao. The Population Effect of Commercial Pigs on the Accuracy of Genomic Selection for Carcass Traits in Duroc Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4943-4951.
[4]	XU Tingting, QI Fenfang, HUANG Shihui, NIU Xi, LI Sheng, RAN Xueqin, WANG Jiafu, XIE Jian. Study of the Polymorphisms of Structural Variation and the Expression of MAP3K4 gene in Xiang Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5046-5055.
[5]	HU Ziping, WANG Ligang, ZONG Wencheng, HOU Renda, SU Yanfang, NIU Naiqi, WANG Lixian, WANG Yuan, ZHNAG Longchao. Genetic Structure Analysis of Jianbai Xiang Pig Population Based on Genomic SNP and ROH [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4117-4125.
[6]	JI Zhengyu, NI Mengru, ZHANG Zhaobo, ZHAO Gan, HUANG Zan, LI Pinghua, HUANG Ruihua, HOU Liming. Research on Adipogenic Capacity and Gene Expression Pattern of in Vitro FAPs Cells Derived from Longissimus Dorsi Muscle of Suhuai Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4126-4142.
[7]	BI Huan, QIN Hai, YUAN Wei, ZHANG Yudan, ZHANG Yiyu, CHEN Wei. Effect of Knocking down TYRP1 Gene on Melanin Production in the Xiang Pig Epidermal Melanocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4143-4153.
[8]	WANG Hui, FENG Baoliang, WU Dan, XIANG Guangming, WANG Nan, MU Yulian, LI Kui, LIU Zhiguo. Research Progress of CD163 Gene and Disease-Resistant Breeding on Porcine Reproductive and Respiratory Syndrome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3127-3138.
[9]	ZHANG Wanfeng, ZHAO Tianzhi, LI Jiao, YOU Ziwei, YANG Yang, CAI Chunbo, GAO Pengfei, CAO Guoqing, GUO Xiaohong, LI Bugao. Study on NR2F2 Gene Regulating Proliferation and Apoptosis of Porcine PK15 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3242-3251.
[10]	LU Chang, DONG Lei, ZHANG Wanfeng, GAO Pengfei, GUO Xiaohong, CAI Chunbo, CAO Guoqing, LI Bugao. Identification and Screening of Single Nucleotide Polymorphism Loci in Jinfen White Pigs Based on Whole Genome Resequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2761-2771.
[11]	YANG Qing, GONG Jing, ZHAO Xueyan, ZHU Xiaodong, GENG Liying, ZHANG Chuansheng, WANG Jiying. Comparison of Array and Resequencing in Pig Genetic Structure Studies [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2772-2782.
[12]	WANG Jinglin, LIU Yangguang, XU Qilong, CHEN Shuo, DENG Zaishuang, CHENG Shiyu, DING Yueyun, ZHENG Xianrui, YIN Zongjun, ZHANG Xiaodong. Genome Structures Variant Analysis and Feature SNPs Screening of Wanyue Black Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2783-2793.
[13]	ZHOU Fuchen, YE Jian, GUO Caijin, ZENG Haiyu, WU Zhenfang, DONG Linsong, CAI Gengyuan. Litter Effect Analysis and Genetic Parameters Estimation of Lean Meat Percentage Trait in Duroc Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2288-2296.
[14]	ZHAO Zhenjian, WANG Shujie, CHEN Dong, JI Xiang, SHEN Qi, YU Yang, CUI Shengdi, WANG Junge, CHEN Ziyang, TANG Guoqing. Population Structure and Genetic Diversity Analysis of Neijiang Pigs Based on Low-coverage Whole Genome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2297-2307.
[15]	TAO Xuan, YANG Xuemei, LIANG Yan, LIU Yihui, WANG Yong, KONG Fanjing, LEI Yunfeng, YANG Yuekui, WANG Yan, AN Rui, YANG Kun, Lü Xuebin, HE Zhiping, GU Yiren. Analysis of Genetic Structure of Conservation Population in Yacha Pig Based on SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2308-2319.