术芩总多糖对免疫低下小鼠脾淋巴细胞增殖与自噬的影响

doi:10.11843/j.issn.0366-6964.2018.12.026

Abstract

Abstract:

To investigate the effect of polysaccharides from ZhuQin Formula (ZQp) on the proliferation and autophagy of spleen lymphocyte in immunocompromised mice, the optimum concentration and time of ZQp on the proliferation of spleen lymphocyte in mice was screened by CCK-8 method. The test was divided into blank group, 3-MA (autophagy inhibitor) group, 3-MA + ZQp group, Western blot was used to determine the effect of ZQp on the expression of autophagy related protein Beclin1 and LC3B in mice under the inhibition of 3-MA; The test was divided into blank group, cyclophosphamide induced immunocompromised model group and model +ZQp group, Western blot was used to determine the effect of ZQp on the expression of autophagy related protein Beclin1 and LC3B in immunocompromised mice. Test results were as follows:1)The ZQp with the concentration of 10^-1μg·mL^-1 acted on mouse spleen lymphocytes for 12 h, and the cell proliferation ability was the strongest; 2)The proliferation ability of spleen lymphocyte in mice with cyclophosphamide immunodeficiency was reduced, and the proliferation of lymphocyte was enhanced by ZQp; 3)When 3-MA inhibited autophagy of spleen lymphocyte, the expression of autophagy related protein Beclin1 decreased, and ZQp could increase the expression of Beclin1; When the expression of autophagy related protein LC3BⅡ/LC3BⅠincreased, ZQp could reduce the expression of LC3BⅡ/LC3BⅠ; 4)The expression of autophagy related protein Beclin1 and LC3BⅡ/LC3BⅠ increased in spleen lymphocytes of immunocompromised mice. ZQp could decrease the expression of Beclin1 and LC3BⅡ/LC3BⅠ protein. The results suggest that:Cyclophosphamide-induced immune dysfunction in mice may be related to the splenic lymphocyte proliferation and development impair and excessive activation of autophagy lead to type Ⅱ programmed cell death. ZQp can inhibit excessive autophagy, enhance the proliferation of lymphocytes, and regulation the immune function by regulating the expression of Beclin1 and LC3BⅡ/LC3BⅠ protein.

CLC Number:

S853.74

PANG Min, ZHU Zhao-rong, QIAO Qian-qian, ZHAN Zhu-yu, LIU Juan. Effect of Polysaccharides from ZhuQin Formula on the Proliferation and Autophagy of Spleen Lymphocyte in Immunocompromised Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2762-2770.

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Effect of Polysaccharides from ZhuQin Formula on the Proliferation and Autophagy of Spleen Lymphocyte in Immunocompromised Mice

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