miR-132-3p靶向UCP2调节绵羊前体脂肪细胞分化的研究

doi:10.11843/j.issn.0366-6964.2017.11.006

Abstract

Abstract:

This study aimed to explore the regulatory mechanism of miR-132-3p targeting UCP2 expression in ovine preadipocytes. In this study, ovine subcutaneous fat cells were used to investigate the mechanism of miR-132-3p targeting UCP2 in ovine preadipocytes. The binding sites of miR-132-3p to UCP2 were predicted by bioinformatics softwares, and were verified via double luciferase report system. Expression of UCP2 mRNA and protein were detected by RT-qPCR and Western blotting, respectively after overexpressing miR-132-3p. The expression of UCP2 mRNA and miR-132-3p were detected by RT-qPCR during differentiation stage in ovine preadipocyte. The results showed that miR-132-3p had binding site to UCP2 3'-UTR and significantly down-regulated the relative fluorescence activity of recombinant double fluorescent plasmid(P<0.05). Overexpressing miR-132-3p significantly down-regulated both UCP2 mRNA(P<0.05) and protein expressions. At last, we found a negative correlation between the expression of miR-132-3p and UCP2 at the middle stage during ovine preadipocytes differentiation. In conclusion, miR-132-3p inhibits UCP2 expression at both levels of mRNA and protein by specifically binding to UCP2 3'-UTR, and thus promotes ovine preadipocytes differentiation to a certain extent.

CLC Number:

S826.2

SHI Tao, YAN Xiao-ru, PAN Yang-yang, JING Jiong-jie, QIAO Li-ying, GUO Yun-li, SHEN Cai-yun, ZHANG Fu-quan, LIU Wen-zhong. Study on the Regulation of miR-132-3p in Ovine Preadipocyte Differentiation by Targeting UCP2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2059-2067.

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Study on the Regulation of miR-132-3p in Ovine Preadipocyte Differentiation by Targeting UCP2

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