LPS通过TLR4影响绵羊输卵管上皮细胞SBD-1的表达

doi:10.11843/j.issn.0366-6964.2016.01.011

Abstract

Abstract:

The aim of this study was to explore the effect of lipopolyscharride (LPS) on induction of sheep beta defensin-1 (SBD-1) in ovine oviduct epithelial cells (OOECs).In this study，OOECs were cultured and then exposed to LPS for up to 24 hours，with a range of concentrations (10 ng•mL^-1-1 mg•mL^-1).qRT-PCR was performed to detect SBD-1 mRNA.The results showed that LPS stimulated the expression of SBD-1 in a time-(maximal at 12 hours) and concentration-(maximal at 100 ng•mL^-1) dependent manner.Moreover，IHC staining showed that SBD-1 was mainly expressed in epithelial cells.Importantly，TLR4 was detected in OOECs and stimulated by LPS.LPS induced SBD-1 mRNA expression was markedly inhibited by blockage of TLR4 activity using anti-TLR4 antibody.In conclusion，these results suggest that LPS might induce the expression of SBD-1 in the ovine oviduct epithelial cells,and the expression are mediated by TLR4.

CLC Number:

S826.2

LI Qi，ZHI Da-fu，YAN Qin，LIU Mo-ning，ZHENG Xin-xin，CAO Gui-fang. LPS Induces SBD-1 Expression via TLR4 in Ovine Oviduct Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 79-84.

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LPS Induces SBD-1 Expression via TLR4 in Ovine Oviduct Epithelial Cells

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