ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (8): 1277-1282.doi: 10.11843/j.issn.0366-6964.2013.08.015

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Isolation and Identification of Reticuloendotheliosis Virus in Wild Birds

JIANG Li-li1, 2, 3, QI Xiao-le1, GAO Yu-long1, DENG Xiao-yun2, CHAI Hong-liang2, ZHANG Li-zhou1, YUN Bing-ling1, QIN Li-ting1, WANG Yong-qiang1, GAO Hong-lei1,WANG Xiao-mei1*, HUA Yu-ping2*   

  1. (1. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001,China; 2.Northeast Forestry University, Harbin 150040, China; 3. Animal Husbandry and Veterinary Medicine, Liaoning Medical Universitye, Jinzhou 121001, China)
  • Received:2012-11-22 Online:2013-08-23 Published:2013-08-23

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Abstract:

The specific primer pairs for amplification of long terminal repeat sequences (LTR) of Reticuloendotheliosis virus (REV) was used in the PCR reaction to amplify the specific nucleic acid sequence of healthy wild birds collected in Jilin province. Positive samples were inoculated on the DF1 cells for virus reproduction.With identification of indirect immunofluorescence assay (IFA), PCR assay, two REV strains were isolated, one from the pintail (Coturnix cotu), another from mallard (Anas platyrhynchos). The two isolates were named DBYR1101 and DBYR1102, respectively. The gp90 gene encoding the most important protective protein was cloned and sequenced. Sequence analysis showed that the gp90 gene based amino acid homology between the two isolates is 99.5%. And they are more identical to the northeast China isolates (the amino acid homology of 99.2% to 99.9%) than to the early Chinese REV isolates HA9901 (94.7% and 94.2%). The two isolates in this study and the northeast China isolates tend to group together in their own distinct phylogenetic clade. The gp90 amino acid homology between DBYR1101 and 3 subtype of REV170A, SNV and CSV was 95.7%, 94% and 98.2% respectively, while DBYR1102 was 95.2%，93.7% and 97.7%. And the gp90 gene between the two isolates and the early southern isolate HA9901 were 94.7% and 94.2%. In addition, the 2 REV strains have a high identity with some REV strains in US and Taiwan, which classified as subtype Ⅲ. This is the first study to investigate the status of wild birds infected with REV, and the results of this paper will not only expands the epidemiological data of REV, but also remind us pay more attention to the role of wild bird migration in the spread of diseases. Meanwhile, it also makes a basis for further researching the pathogenic and immunosuppressive mechanisms.

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