畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (12): 1300-1305.

• 遗传繁育 • 上一篇    下一篇

四川7个地方山羊品种(类群)mtDNA遗传多样性研究

张红平;李利;向德;刘成建;李学伟   

  1. 四川农业大学动物遗传育种与繁殖学重点实验室,雅安 625014
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-12-25 发布日期:2007-12-25

Study on Mitochondrial DNA Genetic Polymorphism of Seven Indigenous Goat Breeds(Groups) in Sichuan

ZHANG Hong-ping;LI Li;XIANG De;LIU Cheng-jian;LI Xue-wei   

  1. Key Laboratory of Animal Genetics,Breeding and Reproduction,Sichuan Agricultural University,Ya’an 625014,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

摘要: 测定了四川7个地方山羊品种(类群)43个个体的mtDNA控制区全序列,结果表明:山羊mtDNA控制区全序列长度为1 212 bp或1 213 bp,A+T含量(59.9%)明显高于G+C含量(40.1%)。共检测到74个变异位点,序列均为中性突变,核苷酸多样度为1.686%,这些差异共定义了27 种单倍型,单倍型多样度为0.966,遗传多样性较为丰富,品种(类群)间存在不同程度的遗传分化。7个地方山羊品种(类群)间的遗传距离变异范围为0.001 7~0.030 6。用MEGA软件的NJ法构建单倍型序列的系统发育无根树,结果表明四川地方山羊品种(类群)有两个母系来源,但是否就对应于角骨 羊(Capra aegagrus)和捻角山羊(Capra falconeri)两个野生祖先,还有待于进一步研究。

关键词: 山羊, mtDNA控制区, 序列变异性, 种群遗传结构, 系统发育

Abstract: Complete sequence of mitochondrial DNA(mtDNA) control region was sequenced in 43 individuals from 7 indigenous goat(Capra hircus)breeds(groups)in Sichuan province. The complete sequence of mtDNA control region was 1 212 or 1 213 base pair, and the content of A+T(59.9%)was higher than that of G+C(40.1%)significantly. 74 sites were polymorphic and all of these sequences were neutral mutation. All sequence polymorphic sites defined 27 haplotypes. High nucleotide diversity(1.868%)and haplotype diversity(0.966) were manifested. These results suggested richer genetic diversity within Sichuan indigenous goat populations and genetic divergence among them. The Kimura 2-parameter distance among breeds(groups)varied from 0.001 7 to 0.030 6. Phylogenetic unrooted trees constructed with neighbourjoining methods showed two maternal origins in Sichuan indigenous goats. More detailed studies should be continued to indicate whether the two maternal origins are Capra aegagrus and Capra falconeri.

Key words: Capra hircus, mitochondrial DNA control region, sequence variability, population genetic structure, phylogenetic