畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (12): 1300-1305.doi:

• 遗传繁育 • 上一篇    下一篇

四川7个地方山羊品种(类群)mtDNA遗传多样性研究

张红平;李利;向德;刘成建;李学伟   

  1. 四川农业大学动物遗传育种与繁殖学重点实验室,雅安 625014
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-12-25 发布日期:2007-12-25

Study on Mitochondrial DNA Genetic Polymorphism of Seven Indigenous Goat Breeds(Groups) in Sichuan

ZHANG Hong-ping;LI Li;XIANG De;LIU Cheng-jian;LI Xue-wei   

  1. Key Laboratory of Animal Genetics,Breeding and Reproduction,Sichuan Agricultural University,Ya’an 625014,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

摘要: 测定了四川7个地方山羊品种(类群)43个个体的mtDNA控制区全序列,结果表明:山羊mtDNA控制区全序列长度为1 212 bp或1 213 bp,A+T含量(59.9%)明显高于G+C含量(40.1%)。共检测到74个变异位点,序列均为中性突变,核苷酸多样度为1.686%,这些差异共定义了27 种单倍型,单倍型多样度为0.966,遗传多样性较为丰富,品种(类群)间存在不同程度的遗传分化。7个地方山羊品种(类群)间的遗传距离变异范围为0.001 7~0.030 6。用MEGA软件的NJ法构建单倍型序列的系统发育无根树,结果表明四川地方山羊品种(类群)有两个母系来源,但是否就对应于角骨 羊(Capra aegagrus)和捻角山羊(Capra falconeri)两个野生祖先,还有待于进一步研究。

Abstract: Complete sequence of mitochondrial DNA(mtDNA) control region was sequenced in 43 individuals from 7 indigenous goat(Capra hircus)breeds(groups)in Sichuan province. The complete sequence of mtDNA control region was 1 212 or 1 213 base pair, and the content of A+T(59.9%)was higher than that of G+C(40.1%)significantly. 74 sites were polymorphic and all of these sequences were neutral mutation. All sequence polymorphic sites defined 27 haplotypes. High nucleotide diversity(1.868%)and haplotype diversity(0.966) were manifested. These results suggested richer genetic diversity within Sichuan indigenous goat populations and genetic divergence among them. The Kimura 2-parameter distance among breeds(groups)varied from 0.001 7 to 0.030 6. Phylogenetic unrooted trees constructed with neighbourjoining methods showed two maternal origins in Sichuan indigenous goats. More detailed studies should be continued to indicate whether the two maternal origins are Capra aegagrus and Capra falconeri.