山羊副流感病毒3型感染MDBK细胞的转录组分析

doi:10.11843/j.issn.0366-6964.2019.02.014

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (2): 364-372.doi: 10.11843/j.issn.0366-6964.2019.02.014

山羊副流感病毒3型感染MDBK细胞的转录组分析

钟纯燕^1,3, 李基棕^1,2*, 毛立¹, 李文良¹, 郝飞¹, 孙敏¹, 刘茂军¹, 主性³, 嵇辛勤³, 肖芳^1,3, 杨蕾蕾¹, 张纹纹¹

1. 江苏省农业科学院兽医研究所, 农业部兽用生物制品工程技术重点实验室, 南京 210014;
2. 临沂大学药学院, 临沂 276000;
3. 贵州大学动物科学学院, 贵阳 550025

收稿日期:2018-07-04 出版日期:2019-02-23 发布日期:2019-02-23
通讯作者: 李基棕,主要从事动物传染病防治和诊断技术研究,Tel:025-84391152,E-mail:lijizong22@sina.com
作者简介:钟纯燕(1992-),女,贵州兴义人,硕士生,主要从事动物传染病防治和诊断技术研究,Tel:025-84391152,E-mail:1462651179@qq.com
基金资助:
国家自然科学基金（31702272）；江苏省自然科学基金（BK2017059）；山东省自然科学基金（ZR2016CP08）；"十三五"国家重点研发计划项目（2016YFD0500908）

Transcriptome Analysis of MDBK Cells Infected with the Caprine Parainfluenza Virus Type 3 JSHA2014-1 Strain

ZHONG Chunyan^1,3, LI Jizong^1,2*, MAO Li¹, LI Wenliang¹, HAO Fei¹, SUN Min¹, LIU Maojun¹, ZHU Xing³, JI Xinqin³, XIAO Fang^1,3, YANG Leilei¹, ZHANG Wenwen¹

1. Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. School of Pharmacy, Linyi University, Linyi 276000, China;
3. College of Animal Science of Guizhou University, Guiyang 550025, China

Received:2018-07-04 Online:2019-02-23 Published:2019-02-23

摘要/Abstract

摘要：

本研究旨在探究山羊副流感病毒3型（CPIV3）感染MDBK细胞后的转录组基因变化情况，丰富CPIV3转录组信息。取1 MOI CPIV3 JSHA2014-1病毒液感染MDBK细胞，设非感染正常细胞为对照，于24 h后收获细胞，提取总RNA，利用Illumina HiSeq^TM 2500对感染组与对照组进行高通量测序，并用测序评估、基因注释等生物信息学方法进行分析。结果显示，差异表达基因共261个，其中表达上调140个，表达下调121个，经RT-qPCR方法验证8个差异表达的干扰素信号通路相关基因，结果与高通量测序一致。进一步GO分类结果显示，差异表达基因主要涉及细胞生物学进程、构成细胞的组分以及实现的分子功能三个方面，KEGG分析显示这些基因参与代谢、生物系统、细胞进程、基因信息进程和环境信息进程。本研究为深入探究CPIV3的致病机制奠定了基础。

Abstract:

To explore the cellular transcriptional gene differentially expressed in MDBK cells infected with caprine parainfluenza virus type 3 (CPIV3), we analyzed the mRNA expression profiles in MDBK cells infected with 1 MOI CPIV3 JSHA2014-1 strain at 24 hpi using high-throughput sequencing. The results indicated that a total of 261 genes were obviously differentially expressed in the infected MDBK cells including 140 up-regulated genes and 121 down-regulated genes. The eight of these genes were verified by RT-qPCR assay, and the results were consistent with those of high-throughput sequencing. GO analysis classification showed that differentially expressed genes were mainly involved in biological processes, cellular components and molecular functions. KEGG analysis shows that the signaling pathways involved in these genes included metabolism-related signaling pathways, biological systems, cellular processes, gene information processes and environmental information processes. This study laid the foundation for further exploring the pathogenesis of CPIV3.

中图分类号:

S852.659.5

钟纯燕, 李基棕, 毛立, 李文良, 郝飞, 孙敏, 刘茂军, 主性, 嵇辛勤, 肖芳, 杨蕾蕾, 张纹纹. 山羊副流感病毒3型感染MDBK细胞的转录组分析[J]. 畜牧兽医学报, 2019, 50(2): 364-372.

ZHONG Chunyan, LI Jizong, MAO Li, LI Wenliang, HAO Fei, SUN Min, LIU Maojun, ZHU Xing, JI Xinqin, XIAO Fang, YANG Leilei, ZHANG Wenwen. Transcriptome Analysis of MDBK Cells Infected with the Caprine Parainfluenza Virus Type 3 JSHA2014-1 Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 364-372.

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山羊副流感病毒3型感染MDBK细胞的转录组分析

Transcriptome Analysis of MDBK Cells Infected with the Caprine Parainfluenza Virus Type 3 JSHA2014-1 Strain

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